Characterization of diacetylenic liposomes as carriers for oral vaccines
Characterization of diacetylenic liposomes as carriers for oral vaccines
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DOI：
10.1016/s0009-3084(02)00190-1
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发表时间：
2003-01-01
影响因子：
3.4
通讯作者：
Taira, MC
中科院分区：
文献类型：
--
作者：
Alonso-Romanowski, S;Chiaramoni, NS;Taira, MC
为了评价脂质体作为口服疫苗载体的作用，研究了聚合脂质体和非聚合脂质体的特性和稳定性。采用1,2-二（10,12-三萜二醇基）-sn-甘油-3磷酸胆碱（dc8,9pc）与饱和1,2-二mimistoyl -sn-甘油-3-磷酸胆碱摩尔比为1:1的混合物。以饱和脂质和不饱和脂质为原料，采用紫外聚合法制备了一种化学改性膜。通过电子显微镜、差示扫描量热法（DSC）和疏水因子（HF）对其进行了表征。对消化道（包括唾液）的稳定性：酸性溶液、胆汁和胰酶与缓冲液pH 7.4进行比较，测量葡萄糖-6-磷酸的释放或牛血浆白蛋白的夹持。聚合后的脂质体的HF和大小进一步增大。与DC8、9PC的数据相比，DSC显示出相分离和更低的Tt。讨论了与体外稳定性相关的主要因素HF，表明聚合和非聚合脂质体可以有效地作为口服给药载体。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
In order to evaluate liposomes as vehicle for oral vaccines the characterization and stability of polymerized and non-polymerized liposomes were examined. Mixtures of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3 phosphocholine) (DC8,9PC) with saturated 1,2-dimiristoyl-sn-glycero-3-phosphocholine in molar ratio 1:1 were used. Saturated and non-saturated lipids were combined to give a chemically modified membrane by UV polymerization derived from DC8,9PC. Characterization was carried out by electronic microscopy, differential scanning calorimetry (DSC) and by hydrophobicity factor (HF). The stability towards the digestive tract (including saliva): acidic solutions, bile and pancreatin are compared to buffer pH 7.4, measuring the release of Glucose-6-phosphate or bovine plasma albumin entrapment. The polymerized liposomes showed further augmentation of the HF and the size. DSC showed phase separation and lower Tt if compared to data obtained for DC8,9PC. The HF, as main factor is discussed in relation to in vitro stability, suggesting that polymerized and non-polymerized liposomes would serve effectively as an oral delivery vehicle. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.