Dynamic analysis of QTLs on tiller number in rice (Oryza sativa L.) with single segment substitution lines
Dynamic analysis of QTLs on tiller number in rice (Oryza sativa L.) with single segment substitution lines
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DOI：
10.1007/s00122-012-1822-x
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发表时间：
2012-06-01
影响因子：
5.4
通讯作者：
Ye, Guoyou
中科院分区：
文献类型：
--
作者：
Liu, Guifu;Zhu, Haitao;Ye, Guoyou
本研究利用12个含有水稻分蘖数数量性状位点的单片段代换系（SSSL），研究了这些QTL的动态表达。以这些单交种和受体花粳籼74为材料，配制78个杂交组合，并与亲本进行两季三密度种植试验。分蘖数在7个发育阶段进行了测量。在每个测定阶段分析了QTL效应，包括主效应（加性、显性和上位性）、QTL与季节的互作效应和QTL与密度的互作效应。12个QTL的加性效应、显性效应、上位性效应及其与季节和密度的互作效应均随生育期呈动态变化。检测到8个QTL至少在一个发育阶段具有显著的加性效应和/或加性×季节和/或加性×密度互作效应，所有QTL均具有显著的显性和上位性效应和/或互作效应。大多数QTL的显性效应远大于加性效应，表现为超显性。每个QTL至少与8个其它QTL互作。这些QTL的加性效应和显性效应以正效应为主，上位性效应以负效应为主。大多数QTL与种植季节和种植密度存在显著的互作效应，但QTL-和-的加性效应、QTL和的显性效应以及14对QTL的上位性效应在不同种植季节间保持稳定，QTL -的显性效应和-/-/和-/-的上位性效应在不同种植密度间基本一致。本研究首次报道了水稻分蘖数QTL的显性和上位性动态，为利用杂种优势和QTL定位改良水稻分蘖数提供了丰富的信息。
Twelve single segment substitution lines (SSSLs) in rice, which contain quantitative trait loci (QTLs) for tiller number detected previously, were used to study dynamic expression of the QTLs in this study. These SSSLs and their recipient, Hua-Jing-Xian 74 (HJX74), were used to produce 78 crossing combinations first, and then these combinations and their parents were grown in two planting seasons with three cropping densities. Tiller number was measured at seven developmental stages. QTL effects including main effects (additive, dominance and epistasis), QTL x season and QTL x density interaction effects were analyzed at each measured stage. The additive, dominant and epistatic effects of the 12 QTLs as well as their interaction effects with the seasons and with the densities all display dynamic changes with the development. Eight QTLs are detected with significant additive effects and/or additive x season and/or additive x density interaction effects at least at one developmental stage, and all QTLs have significant dominant and epistatic effects and/or interaction effects involved in. For most of the QTLs dominant effects are much bigger than additive effects, showing overdominance. Each QTL interacts at least with eight other QTLs. Additive and dominant effects of these QTLs are mostly positive while epistatic effects are negative and minor. Most of the QTLs show significant interactions with planting seasons and cropping densities, but the additive effects of QTLs - and -, the dominant effects of QTL and , and the epistatic effects of 14 pairs of QTLs are stable across seasons and the dominant effect of QTL - and the epistatic effects of QTL pairs -/--/ and -/- are nearly consistent across cropping densities. This paper is the first report of dynamics on dominances and epistasis of QTLs for tiller number in rice and provides abundant information, which is useful to improve rice tiller number via heterosis and/or QTL pyramiding.