Solubility and stability of cytochrome c in hydrated ionic liquids:: Effect of oxo acid residues and kosmotropicity
Solubility and stability of cytochrome c in hydrated ionic liquids:: Effect of oxo acid residues and kosmotropicity
复制标题
DOI：
10.1021/bm070041o
复制
发表时间：
2007-07-01
影响因子：
6.2
通讯作者：
Ohno, Hiroyuki
中科院分区：
文献类型：
--
作者：
Fujita, Kyoko;MacFarlane, Douglas R.;Ohno, Hiroyuki
通过向亲水性离子液体中加入适量的水制备了水合离子液体。一些水合离子液体对蛋白质表现出很好的增溶能力，并保持了离子液体的基本性质。细胞色素c（cyt c）的溶解度取决于组分离子的结构。当组分阴离子具有含氧酸残基时，所得水合离子液体相当好地增溶细胞色素c。在这种水合离子液体中，细胞色素c的结构和活性受到组分离子的亲液性的影响。我们从具有不同亲液性的各种离子合成了离子液体，包括磷酸二氢盐（dhp），磷酸二丁酯，乙酸盐，乳酸盐和甲磺酸盐作为阴离子。溶解的细胞色素c的活性取决于组分离子的亲液性排列。当溶解在水合胆碱DHP中时，CytC没有显示出结构变化，并保持其活性，所述水合胆碱DHP是离液序列高的阳离子和离液序列低的阴离子的优异组合。此外，溶解在水合胆碱dhp中的cyt c保持天然状态，并在室温下储存18个月后仍有活性。
Hydrated ionic liquids (ILs) were prepared by adding appropriate amounts of water to hydrophilic ILs. Some hydrated ILs show excellent solubilizing ability for proteins, keeping the basic properties of ILs. The solubility of cytochrome c (cyt c) depended on the structure of the component ions. When component anions have oxo acid residues, the resulting hydrated ILs solubilize cyt c quite well. In such hydrated ILs, the structure and activity of cyt c is influenced by the kosmotropicity of the component ions. We synthesized ILs from various ions having different kosmotropicity, including dihydrogen phosphate (dhp), dibutylphosphate, acetate, lactate, and methanesulfonate as anions. The activity of the dissolved cyt c depends on the permutations of kosmotropicity of the component ions. cyt c shows no structural change and retains its activity when dissolved in the hydrated choline dhp, which is an excellent combination of chaotropic cation and kosmotropic anion. Furthermore, cyt c dissolved in the hydrated choline dhp remained in a native state and was active after 18 months of storage at room temperature.