Selective regulation of trypsin gene expression by calcium and by glucose starvation in a rat exocrine pancreas cell line.
Selective regulation of trypsin gene expression by calcium and by glucose starvation in a rat exocrine pancreas cell line.
复制标题
大鼠外分泌胰腺细胞系中钙和葡萄糖饥饿对胰蛋白酶基因表达的选择性调节。
DOI：
10.1073/pnas.83.12.4292
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Stratowa,C;Rutter,WJ
用钙离子载体A23187处理大鼠胰腺腺泡细胞系AR4-2J，在几小时内选择性地增加胰酶mRNA的稳态水平。添加促进肿瘤的佛波酯佛波酯12-肉豆蔻酸盐13-醋酸酯会增强钙诱导的增加。其他被测试的外分泌胰腺基因的mRNA水平下降。利用与氯霉素乙酰转移酶(CAT)基因编码序列连锁的胰酶和糜蛋白酶基因5‘侧翼区的DNA转染实验证实了上述结果。在钙诱导的细胞中，观察到转染了胰蛋白酶结构体的CAT活性增加，而胰凝乳酶结构体显示CAT活性降低。葡萄糖饥饿的AR4-2J细胞类似地引起胰酶mRNA的选择性增加。这种对胰蛋白酶的选择性调节可能反映了它作为其他酶原物种的关键激活剂的作用。
Treatment of the rat pancreatic acinar cell line AR4-2J with the calcium ionophore A23187 selectively increases, within a few hours, the steady-state level of trypsin mRNA. Addition of the tumor-promoting phorbol ester phorbol 12-myristate 13-acetate potentiates the calcium-induced increase. The mRNA level of the other tested exocrine pancreatic genes decreases. These results were confirmed by DNA transfection experiments, using the 5' flanking region of the trypsin and chymotrypsin genes linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. In calcium-induced cells transfected with the trypsin constructs, an increase in CAT activity was observed, whereas the chymotrypsin constructs revealed a decreased CAT activity. Glucose starvation of AR4-2J cells similarly elicited a selective increase in trypsin mRNA. This selective regulation of trypsin may reflect its role as the key activator of the other zymogen species.