Non-invasive imaging for studying anti-angiogenic therapy effects.
Non-invasive imaging for studying anti-angiogenic therapy effects.
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DOI：
10.1160/th12-10-0721
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发表时间：
2013-03
影响因子：
6.7
通讯作者：
Kiessling F
中科院分区：
文献类型：
--
作者：
Ehling J;Lammers T;Kiessling F
非侵入性成像在临床前和临床癌症研究中发挥着新兴作用，并具有很大的潜力来改善新药的临床转化。本文总结和讨论了肿瘤血管生成成像和抗血管生成治疗效果监测的工具和方法。在这种情况下，建议使用微计算机断层扫描（μCT）来可视化和量化功能性肿瘤血管的微结构。对比增强超声（US）和磁共振成像（MRI）是评估功能性血管参数（如灌注和相对血容量）的有利工具。这些功能参数已显示在肿瘤大小出现变化之前的早期阶段指示抗血管生成治疗反应。对于肿瘤表征，肿瘤血管的分子特征（例如受体表达）的成像可能具有甚至更高的诊断潜力，并且已被证明也非常适合于治疗监测。在这种情况下，使用靶向微泡的超声目前在临床试验中作为血管生成内皮细胞分子表征的重要工具进行评价。优选用于血管及其周围基质的分子成像的其它模态是光声成像（派）、近红外荧光光学成像（OI）、MRI、正电子发射断层扫描（PET）和单光子发射计算机断层扫描（SPECT）。如果需要非常高的灵敏度，和/或如果分子靶标难以接近，则后两者特别有用。在多模式成像设置中仔细考虑不同成像模式的利弊，可以对肿瘤血管的（微观）形态、功能和分子调控进行全面的纵向评估。
Noninvasive imaging plays an emerging role in preclinical and clinical cancer research and has high potential to improve clinical translation of new drugs. This article summarizes and discusses tools and methods to image tumor angiogenesis and monitor anti-angiogenic therapy effects. In this context, micro-computed tomography (μCT) is recommended to visualize and quantify the micro-architecture of functional tumor vessels. Contrast-enhanced ultrasound (US) and magnetic resonance imaging (MRI) are favorable tools to assess functional vascular parameters, such as perfusion and relative blood volume. These functional parameters have been shown to indicate anti-angiogenic therapy response at an early stage, before changes in tumor size appear. For tumor characterization, the imaging of the molecular characteristics of tumor blood vessels, such as receptor expression, might have an even higher diagnostic potential and has been shown to be highly suitable for therapy monitoring as well. In this context, US using targeted microbubbles is currently evaluated in clinical trials as an important tool for the molecular characterization of the angiogenic endothelium. Other modalities, being preferably used for molecular imaging of vessels and their surrounding stroma, are photoacoustic imaging (PAI), near-infrared fluorescence optical imaging (OI), MRI, positron emission tomography (PET) and single photon emission computed tomography (SPECT). The latter two are particularly useful if very high sensitivity is needed, and/or if the molecular target is difficult to access. Carefully considering the pros and cons of different imaging modalities in a multimodal imaging setup enables a comprehensive longitudinal assessment of the (micro)morphology, function and molecular regulation of tumor vessels.