Exposure to diclofop-methyl induces cardiac developmental toxicity in zebrafish embryos
Exposure to diclofop-methyl induces cardiac developmental toxicity in zebrafish embryos
复制标题
接触甲基敌草灵会导致斑马鱼胚胎出现心脏发育毒性。
DOI：
10.1016/j.envpol.2020.113926
复制
发表时间：
2020-04-01
影响因子：
8.9
通讯作者：
Lu, Huiqiang
中科院分区：
文献类型：
--
作者：
Cao, Zigang;Huang, Yong;Lu, Huiqiang
Diclofop-methyl (DM) is one of the most widely used herbicides in agriculture production and has been frequently detected in both freshwater and environments, even agricultural products. However, the potential toxic effects of DM on organisms and the underlying mechanisms are still poorly understood. In this study, we utilized zebrafish to evaluate the toxicity of DM during the cardiovascular developmental process. Exposure of zebrafish embryos to 0.75, 1.0 and 1.25 mg/L DM induced cardiac defects, such as pericardial edema, slow heart rate and long SV-BA distance but the vascular development in zebrafish larvae was not influenced by DM treatment. The expression of cardiac-related genes were disordered and DM exposure initiated disordering cardiogenesis from the period of precardiac mesoderm formation. Moreover, the apoptosis and proliferation of cardiomyocytes were not influenced but the levels of oxidative stress were upregulated by DM exposure. Fullerenes and astaxanthin was able to rescue cardiac defects caused by DM via downregulating oxidative stress. Wnt signaling was downregulated after DM treatment and activation of Wnt signaling could rescue cardiac defects. Therefore, our results suggest that DM has the potential to induce cardiac developmental toxicity through upregulation of Wnt-Mediated (reactive oxygen species) ROS generation in zebrafish larvae. (C) 2020 Elsevier Ltd. All rights reserved.
