Tuning the Viscoelasticity of Peptide Vesicles by Adjusting Hydrophobic Helical Blocks Comprising Amphiphilic Polypeptides
Tuning the Viscoelasticity of Peptide Vesicles by Adjusting Hydrophobic Helical Blocks Comprising Amphiphilic Polypeptides
复制标题
DOI：
10.1021/acs.langmuir.7b00289
复制
发表时间：
2017-06-06
期刊：
影响因子：
3.9
通讯作者：
Kimura, Shunsaku
中科院分区：
文献类型：
--
作者：
Kim, Cheol Joo;Ueda, Motoki;Kimura, Shunsaku
[GRAPHIC]poly（sarcosine）-B-（L-Val-Aib）（6）和poly（sarcosine）-B-（L-Leu-Aib）（6）的两亲性嵌段多肽及其立体异构体在水中自组装。聚（肌氨酸）-B-（L-Leu-Aib）（6）与聚（肌氨酸）-b-聚（D-Leu-Aib）（6）、聚（肌氨酸）-b-聚（L-Val-Aib）（6）或聚（肌氨酸）-B-（D-Val-Aib）（6）的三种二元体系产生直径为200 nm的囊泡。在水中使用AFM通过纳米压痕法评估囊泡膜的粘弹性。聚（肌氨酸）-B-（L-Leu-Aib）（6）/聚（肌氨酸）-b-聚（D-Leu-Aib）（6）囊泡的弹性比蛋黄脂质体的弹性高11倍，但在基于Leu和Val的两亲性多肽的结合中降低。膜的弹性被发现是可调的螺旋布洛克在立体复合物的形成和螺旋之间的侧链在分子组装体中的相互交错的适当组合。
Amphiphilic block polypeptides of[GRAPHIC]poly(sarcosine)-b-(L-Val-Aib)(6) and poly(sarcosine)-b-(L-Leu-Aib)(6) and their steteoisomers were self-assembled in water. Three kinds of binary systems of poly(sarcosine)-b-(L-Leu-Aib)(6) with poly(sarcosine)-b-poly(D-Leu-Aib)(6), poly(sarcosine)-b-poly(L-Val-Aib)(6), or poly(sarcosine)-b-(D-Val-Aib)(6) generated vesicles of 200 nm diameter. The viscoelasticity of the vesicle membranes was evaluated by the nanoindentation method using AFM in water. The elasticity of the poly(sarcosine)-b-(L-Leu-Aib)(6)/poly(sarcosine)-b-poly(D-Leu-Aib)(6) vesicle was 11 fold higher than that of the egg yolk liposome but decreased in coinbinations,of the Leu- and Val-based amphiphilic polypeptides. The membrane elasticity is found to be adjustable by a suitable combination of helical brocks in terms of stereocomplex formation and the interdigitation of side chains among helices in the molecular assemblies.