Hydrophilic magnetic latex for nucleic acid extraction, purification and concentration
Hydrophilic magnetic latex for nucleic acid extraction, purification and concentration
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DOI：
10.1016/s0304-8853(00)01240-3
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发表时间：
2001-04-01
影响因子：
2.7
通讯作者：
Herve, C
中科院分区：
文献类型：
--
作者：
Elaïssari, A;Rodrigue, M;Herve, C
采用沉淀聚合法制备了壳层含聚N-异丙基丙烯酰胺的核壳磁性乳胶粒。通过控制介质的pH值和盐度，颗粒表面的阳离子性质有利于核酸的吸附-解吸。给出了核酸的浓缩过程，并以DNA为模型进行了验证。(C)2001 Elsevier Science B.V.保留所有权利。
Core-shell magnetic latex particles bearing poly(N-isopropylacrylamide) in the shell were prepared by encapsulation of magnetic core using a precipitation polymerization process. The cationic character of the particles' surface is favorable for nucleic acid adsorption-desorption by controlling the pH and salinity of the medium. The concentration process of nucleic acids was presented and proven using DNA as a model. (C) 2001 Elsevier Science B.V. All rights reserved.