Changes in apoptosis-like programmed cell death and viability during the cryopreservation of pollen from Paeonia suffruticosa
Changes in apoptosis-like programmed cell death and viability during the cryopreservation of pollen from Paeonia suffruticosa
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DOI：
10.1007/s11240-019-01732-1
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发表时间：
2019-11
期刊：
影响因子：
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通讯作者：
R. Ren;Zedi Li;Hao Zhou;Lingling Zhang;Xue-ru Jiang;Yan Liu
中科院分区：
文献类型：
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作者：
R. Ren;Zedi Li;Hao Zhou;Lingling Zhang;Xue-ru Jiang;Yan Liu
低温保存后的花粉活力变化趋势多种多样，多数花粉活力下降。凋亡样程序性细胞死亡（AL-PCD）在改变细胞活力中的作用尚不完全清楚。AL-PCD是一种重要的细胞生物活性，对植物的正常发育、维持体内平衡和抵抗各种环境因素的干扰起着关键作用。然而，液氮保存后花粉活力的变化与AL-PCD之间的关系鲜有报道。本研究结果表明，6个品种的牡丹花粉经过低温保存后，花粉活力发生了不同程度的变化，6个品种花粉中AL-PCD的含量也有所不同。当品种活力显著高于新鲜花粉或与新鲜花粉活力无显著差异时，细胞内活性氧（ROS）生成变化不显著，caspase-3样酶活性相对较低。当低温保存后的品种活力显著低于新鲜花粉时，活性氧的产生显著增加，caspase-3样酶活性高于对照水平。3种花粉活力变化类型的花粉凋亡率均升高，且相关分析结果显示凋亡率与花粉活力呈显著负相关（P < 0.05）。然而，这6个品种的花粉中AL-PCD后期指标的DNA片段化特征不明显。因此，我们认为AL-PCD发生在花粉低温保存过程中，并与低温保存后花粉活力的差异有关。
Pollen after cryopreservation has a variety of change trends in viability, with most pollens showing decreased viability. The role of apoptosis-like programmed cell death (AL-PCD) in changing viability is not exactly clear. AL-PCD is an important biological activity of cells that plays a key role in the normal development of plants, maintenance of homeostasis and resistance to interference from various environmental factors. However, a relationship between the diverse changes in pollen viability after liquid nitrogen (LN) preservation and AL-PCD has rarely been reported. The results of this study show that after cryopreservation, the viability of pollen from six cultivars ofPaeonia suffruticosapresents different changes, and the occurrence of AL-PCD in pollen of these six cultivars varies. When the viability of the cultivars was significantly higher than that of fresh pollen or the viability showed no significant difference from that of fresh pollen, intracellular reactive oxygen species (ROS) generation was not significantly changed and the activity of the caspase-3-like enzyme was relatively low. When the viability of the cultivars after cryopreservation was significantly lower than that of fresh pollen, ROS generation significantly increased and the caspase-3-like enzymatic activity was higher than the control level. The pollen apoptosis rate increased for all three viability change types, and correlation analysis results demonstrated a significant negative correlation between the apoptosis rate and pollen viability (P < 0.05). However, the DNA fragmentation of AL-PCD late indicators was not characterized in the pollen of these six cultivars. Based on these findings, we concluded that AL-PCD occurs during pollen cryopreservation and is related to the differences in pollen viability after cryopreservation.