The regulatory role of divalent cations in human granulocyte chemotaxis. Evidence for an association between calcium exchanges and microtubule assembly.
The regulatory role of divalent cations in human granulocyte chemotaxis. Evidence for an association between calcium exchanges and microtubule assembly.
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DOI：
10.1083/jcb.62.3.594
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发表时间：
1974-09
期刊：
影响因子：
--
通讯作者：
Rosenthal AS
中科院分区：
文献类型：
--
作者：
Gallin JI;Rosenthal AS
最佳的人粒细胞趋化性已被证明需要钙和镁。暴露于三种不同的趋化因子（C5a，激肽释放酶，和可透析的转移因子）的粒细胞产生了快速的钙释放，抑制钙的摄取，并与钙的细胞质和颗粒部分的转移。秋水仙碱，叠氮化钠，细胞松弛素B，在浓度抑制趋化性，也抑制钙的释放，而低浓度的细胞松弛素B，增强趋化性，也增强钙的释放。微管组装在悬浮于C5a中的细胞中（没有趋化梯度）和通过微孔过滤器主动迁移的细胞中均可见。这些数据表明，微管组装的调节，至少在一定程度上，由细胞质钙的水平。有人提出，微管的不对称组装可能有助于在赋予净矢量的运动在趋化性。
Optimal human granulocyte chemotaxis has been shown to require both calcium and magnesium. Exposure of granulocytes to three different chemotactic factors (C5a, kallikrein, and dialyzable transfer factor) yielded a rapid calcium release, depressed calcium uptake, and was associated with a shift of calcium out of the cytoplasm and into a granule fraction. Colchicine, sodium azide, and cytochalasin B, in concentrations that inhibited chemotaxis, also inhibited calcium release while low concentrations of cytochalasin B, which enhanced chemotaxis, also enhanced calcium release. Microtubule assembly was visualized both in cells suspended in C5a without a chemotactic gradient and in cells actively migrating through a Micropore filter. The data suggest microtubule assembly is regulated, at least, in part, by the level of cytoplasmic calcium. It is proposed that asymmetric assembly of microtubules may be instrumental in imparting the net vector of motion during chemotaxis.