The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24
The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24
复制标题
氧化还原酶 DsbA1 通过干扰荧光假单胞菌 2P24 中 Gcd 的功能，对 2,4-二乙酰基间苯三酚生物合成产生负面影响。
DOI：
10.1186/s12866-020-1714-1
复制
发表时间：
2019-09
期刊：
影响因子：
4.2
通讯作者：
Zhang Li-Qun
中科院分区：
文献类型：
--
作者：
Zhang Bo;Zhao Hui;Wu Xiaogang;Zhang Li-Qun
背景
Background
The polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by Pseudomonas fluorescens 2P24, is positively regulated by the GacS-GacA two-component system.
Results
Here we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in P. fluorescens. Our data showed that mutation of dsbA1 caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production.
Conclusions
Our results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in P. fluorescens 2P24.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
10.1094/mpmi-18-0260
发表时间：
2005-03
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Maria Péchy-Tarr;M. Bottiglieri;S. Mathys;K. Lejbølle;U. Schnider-Keel;M. Maurhofer;C. Keel
通讯作者：
Maria Péchy-Tarr;M. Bottiglieri;S. Mathys;K. Lejbølle;U. Schnider-Keel;M. Maurhofer;C. Keel
影响因子：
6.7
作者：
Qing Yan;Xiaogang Wu;Hai-Lei Wei;Hui‐min Wang;Li-qun Zhang
通讯作者：
Qing Yan;Xiaogang Wu;Hai-Lei Wei;Hui‐min Wang;Li-qun Zhang
影响因子：
1.8
作者：
Dubuis, Christophe;Keel, Christoph;Haas, Dieter
通讯作者：
Haas, Dieter
胆盐诱导的分子间二硫键形成激活霍乱弧菌毒力
DOI：
10.1073/pnas.1218039110
发表时间：
2013-02-05
影响因子：
11.1
作者：
Yang, Menghua;Liu, Zhi;Zhu, Jun
通讯作者：
Zhu, Jun