Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone
Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone
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DOI：
10.1016/j.bone.2003.11.003
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发表时间：
2004-03-01
期刊：
影响因子：
4.1
通讯作者：
Schaffler, MB
中科院分区：
文献类型：
--
作者：
Akkus, O;Adar, F;Schaffler, MB
骨量和结构的测量需要补充物理化学和成分测量，以更好地评估骨折风险。在目前的研究中，我们研究了矿物晶体的物理化学性质对衰老大鼠皮质骨组织和器官水平机械功能的影响。我们的假设是，与年龄相关的矿物晶体物理化学性质的变化与皮质骨组织的弹性变形能力受损有关。用拉曼光谱研究了青年(3月龄)、中年(8月龄)和老年(24月龄)雌性SD大鼠股骨矿化(矿物和有机基质的相对含量)、碳酸盐离子在磷酸盐位置的取代(B型碳酸盐取代)和矿物结晶度(晶格有序性)随年龄的变化。用组织形态计量学方法测量股骨的横截面特性，测量骨干中段的面积和转动惯量，并通过三点弯曲实验测量股骨的弹性变形能力。结果表明，随着年龄的增长，老年大鼠的弹性变形能力在组织和器官水平上均明显受损。在弹性变形能力受损的同时，为了支持我们的假设，我们发现矿化度的增加、结晶度的增加和B型碳酸盐替代的增加与弹性变形能力随年龄的下降显著相关。我们的结论是，骨量的测量需要补充反映矿物晶体的物理化学状态的测量，以改进骨折易感性的评估。(C)2004 Elsevier Inc.保留所有权利。
The measures of bone mass and architecture need to be supplemented with physicochemical and compositional measures for better assessment of fracture risk. in the current studies, we investigated the effects of physicochemical properties of mineral crystals on tissue and organ-level mechanical function of aging rat cortical bone. Our hypothesis was that age-related changes in physicochemical properties of mineral crystals are related to impaired elastic deformability of cortical bone tissue. Raman micro spectroscopy was used to investigate the age-related changes in mineralization (relative amounts of mineral and organic matrix), the substitution of carbonate ions in phosphate positions (type-B carbonate substitution) and mineral crystallinity (the orderliness of crystal lattice) of femurs from young adult (3-month old), middle-aged (8-month old) and aged (24-month old) female Sprague-Dawley rats. Cross-sectional properties, the area and the moment of inertia at the mid-diaphysis, were histomorphometrically quantified and the elastic deformation capacity of femurs was quantified via three-point bending tests. It was observed that the elastic deformation capacity of aged rats was significantly impaired both at the tissue and the organ levels with increasing age. In parallel with this impairment in the elastic deformability and in support of our hypothesis, we found that increasing mineralization, increasing crystallinity and increasing type-B carbonate substitution were significantly correlated with decreasing elastic deformation capacity with age. We conclude that the measure of bone mass needs to be supplemented with measures reflecting the physicochemical status of mineral crystals for improved assessment of fracture susceptibility. (C) 2004 Elsevier Inc. All rights reserved.