Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement.
Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement.
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DOI：
10.1021/acs.analchem.5b04758
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发表时间：
2016-01
影响因子：
7.4
通讯作者：
Yuji Oshikawa;K. Furuta;Satoshi Tanaka;A. Ojida
中科院分区：
文献类型：
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作者：
Yuji Oshikawa;K. Furuta;Satoshi Tanaka;A. Ojida
肥大细胞在各种刺激触发的脱粒后分泌组胺。在此，我们报告了一种新的检测肥大细胞脱颗粒的方法，使用荧光探针能够检测释放的组胺。该探针被设计为钴（II）配合物的花青染料，这表明打开荧光信号的基础上组胺诱导的协调位移机制。利用细胞表面锚定的荧光探针进行荧光成像，能够实时检测各种促分泌素诱导的肥大细胞脱颗粒。
Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.