THE ROLE OF HUMAN SALIVARY ACIDIC PROLINE-RICH PROTEINS IN THE FORMATION OF ACQUIRED DENTAL PELLICLE INVIVO AND THEIR FATE AFTER ADSORPTION TO THE HUMAN-ENAMEL SURFACE
THE ROLE OF HUMAN SALIVARY ACIDIC PROLINE-RICH PROTEINS IN THE FORMATION OF ACQUIRED DENTAL PELLICLE INVIVO AND THEIR FATE AFTER ADSORPTION TO THE HUMAN-ENAMEL SURFACE
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DOI：
10.1016/0003-9969(83)90022-5
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发表时间：
1983-01-01
影响因子：
3
通讯作者：
MADAPALLIMATTAM, G
中科院分区：
文献类型：
--
作者：
BENNICK, A;CHAU, G;MADAPALLIMATTAM, G
通过酸提取去除在腭器具上佩戴1分钟至24小时的人牙釉质碎片上形成的薄膜，并测定蛋白质和酸性富含脯氨酸的蛋白质的总量。由富含脯氨酸的蛋白质构成的总提取蛋白质的百分比在形成的第1小时期间增加至约百分之三十七在1小时和24小时内形成的薄膜之间的总脯氨酸丰富的蛋白质几乎没有差异，但有一个逐渐降解的蛋白质。蛋白质的N-或C-末端部分没有优先保留。24小时的旧获得性牙菌膜提取< 0.1% proline-rich proteins and pellicles >物显示吸附的富含脯氨酸的蛋白质降解;没有迹象表明含有已知生物活性的N-末端部分比C-末端部分保留更大的程度。
The pellicles formed on fragments of human dental enamel worn on a palatal appliance for 1 min to 24 h were removed by acid extraction and the total amounts of protein and of acidic proline-rich proteins were determined. The percentage of total extracted protein constituted by the proline-rich proteins increased during the 1st h of formation to .apprx. 37%. Little difference was seen in total proline-rich protein between pellicles formed in a 1- and a 24-h period; but there was a gradual degradation of the proteins. There was no preferential retention of the N- or C-terminal parts of the proteins. Extracts of old acquired dental pellicle contained < 0.1% proline-rich proteins and pellicles > 24 h old showed degradation of the adsorbed proline-rich proteins; there is no indication that the N-terminal part which contains the known biological activities was retained to a greater extent than the C-terminal part.