An Infection-Relevant Transcriptomic Compendium for Salmonella enterica Serovar Typhimurium
An Infection-Relevant Transcriptomic Compendium for Salmonella enterica Serovar Typhimurium
复制标题
DOI：
10.1016/j.chom.2013.11.010
复制
发表时间：
2013-12-11
影响因子：
30.3
通讯作者：
Hinton, Jay C. D.
中科院分区：
文献类型：
--
作者：
Kroeger, Carsten;Colgan, Aoife;Hinton, Jay C. D.
细菌转录网络由数百个转录因子和数千个启动子组成。然而，细菌病原体中转录的真正复杂性和感染期间遇到的环境的影响仍有待确定。我们提出了一种简化的方法，使用基于RNA-seq的转录组学分析22种不同的感染相关的环境条件，在细菌中进行全局启动子识别。将单个RNA样品组合以在两次RNA-seq运行中鉴定3，838个沙门氏菌血清型鼠伤寒沙门氏菌启动子中的大多数。单个体外条件刺激了特征性的转录特征，22种条件的套件诱导了所有S.鼠伤寒杆菌基因。我们强调了诱导沙门氏菌致病岛的环境条件，并提出了280个sRNA的小RNA表达景观。这个公开可用的纲要环境控制表达的每一个转录功能的S。鼠伤寒沙门氏菌是细菌研究界的有用资源。
Bacterial transcriptional networks consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen and the effect of the environments encountered during infection remain to be established. We present a simplified approach for global promoter identification in bacteria using RNA-seq-based transcriptomic analyses of 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined to identify most of the 3,838 Salmonella enterica serovar Typhimurium promoters in just two RNA-seq runs. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced transcription of 86% of all S. Typhimurium genes. We highlight the environmental conditions that induce the Salmonella pathogenicity islands and present a small RNA expression landscape of 280 sRNAs. This publicly available compendium of environmentally controlled expression of every transcriptional feature of S. Typhimurium constitutes a useful resource for the bacterial research community.