Inhibition of spontaneous and photo-enhanced lipid peroxidation in mouse epidermal microsomes by epicatechin derivatives from green tea.
Inhibition of spontaneous and photo-enhanced lipid peroxidation in mouse epidermal microsomes by epicatechin derivatives from green tea.
复制标题
绿茶中的表儿茶素衍生物抑制小鼠表皮微粒体中自发和光增强的脂质过氧化。
DOI：
10.1016/0304-3835(94)90063-9
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发表时间：
1994
期刊：
影响因子：
9.7
通讯作者：
Mukhtar,H
中科院分区：
文献类型：
--
作者：
Katiyar,SK;Agarwal,R;Mukhtar,H
近年来，绿色茶在几种动物肿瘤生物测定系统中显示出对化学致癌和光致癌的保护作用。有人认为，绿色茶广泛的抗癌特性可能是由于其中存在的表儿茶素的抗氧化作用。在这项研究中，我们评估了这些表儿茶素衍生物（ECD）-即（−）-表没食子儿茶素（EGC），（−）-表儿茶素没食子酸酯（ECG），（−）-表没食子儿茶素-3-没食子酸酯（EGCG）和（−）-表儿茶素（EC）是否抑制小鼠表皮微粒体中自发和光增强的脂质过氧化（LPO）。结果表明，EGCG、EGC和ECG对Fe 3+ ADP支持的LPO有明显的抑制作用（P <0.0 5 ~<0.0 0 0 1）。有趣的是，这些表儿茶素衍生物中的每一种也有效地抑制通过在硅酞菁和650 nm照射的存在下孵育表皮微粒体产生的光增强的LPO。然而，在等摩尔的基础上，EGCG，这也是GTP的主要成分，显示最大的抑制作用相比，其他ECD。综上所述，我们的研究结果提供了直接的证据，抗氧化性能的ECD，并建议这种效果可能有助于抗癌（特别是抗皮肤肿瘤）促进作用的绿色茶。
In recent years green tea has been shown to afford protection against chemical-and photo-carcinogenesis in several animal tumor bioassay systems. It has been suggested that the wide range of anticarcinogenic properties of green tea may be due to the antioxidant effect of epicatechins present therein. In this study, we assessed whether these epicatechin derivatives (ECDs)—namely (−)-epigallocatechin (EGC),(−)-epicatechin gallate (ECG),(−)-epigallocatechin-3-gallate (EGCG) and (−)-epicatechin (EC) inhibit spontaneous and photo-enhanced lipid peroxidation (LPO) in mouse epidermal microsomes. Our data indicate that significant inhibition (significance levels for P,< 0.05 to< 0.0001) was evident by EGCG, EGC and ECG in Fe 3+ ADP supported LPO. Interestingly each of these epicatechin derivatives was also effective in inhibiting photo-enhanced LPO generated by incubating epidermal microsomes in the presence of silicon phthalocyanine and 650 nm irradiation. However, at equimolar basis, EGCG, which is also the major constituent in GTP, showed maximum inhibitory effects compared to other ECDs. Taken together, our results provide the direct evidence for the antioxidant property of ECDs, and suggest that such an effect may contribute towards anticarcinogenic (specifically anti-skin tumor) promoting effects of green tea.