Precise Spatially Selective Photothermolysis Using Modulated Femtosecond Lasers and Real-time Multimodal Microscopy Monitoring.
Precise Spatially Selective Photothermolysis Using Modulated Femtosecond Lasers and Real-time Multimodal Microscopy Monitoring.
复制标题
作者：
Huang Y;Lui H;Zhao J;Wu Z;Zeng H
激光在皮肤病治疗和美容手术中的成功应用主要基于传统的选择性光疗法的原理，该原理强烈依赖于治疗目标与其周围环境之间的吸收差异。然而，当吸收的差异不充分时，由于不加选择和非特异性组织加热，会发生附带损伤。为了处理这样的情况下，我们介绍了一种新的空间选择性光热方法的基础上多光子吸收，其中的辐射能量的紧密聚焦的近红外飞秒激光束可以在空间上通过瞄准激光焦点感兴趣的目标。我们构建了一个多模态光学显微镜来执行和监测空间选择性光热。我们证明，通过选择适当的飞秒激光曝光与多模态光学显微镜监测在真实的时间，同时保持周围组织的完整性，实现了目标组织的精确改变。
The successful application of lasers in the treatment of skin diseases and cosmetic surgery is largely based on the principle of conventional selective photothermolysis which relies strongly on the difference in the absorption between the therapeutic target and its surroundings. However, when the differentiation in absorption is not sufficient, collateral damage would occur due to indiscriminate and nonspecific tissue heating. To deal with such cases, we introduce a novel spatially selective photothermolysis method based on multiphoton absorption in which the radiant energy of a tightly focused near-infrared femtosecond laser beam can be directed spatially by aiming the laser focal point to the target of interest. We construct a multimodal optical microscope to perform and monitor the spatially selective photothermolysis. We demonstrate that precise alteration of the targeted tissue is achieved while leaving surrounding tissue intact by choosing appropriate femtosecond laser exposure with multimodal optical microscopy monitoring in real time.