Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system
Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system
复制标题
溶酶杆菌利用 IV 型分泌系统拮抗土传细菌
DOI：
10.1111/1462-2920.15662
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发表时间：
2021-07-14
影响因子：
5.1
通讯作者：
Qian, Guoliang
中科院分区：
文献类型：
--
作者：
Shen, Xi;Wang, Bingxin;Qian, Guoliang
土壤微生物组包括许多微生物物种，它们不断相互作用。在多种相互作用的模式中，细胞-细胞杀伤可能在塑造微生物组组成中发挥关键作用。细菌利用各种分泌系统来抵御其他微生物，致病菌中的IV型分泌系统（T4 SS）仅在最近才被证明作为接触依赖性的细菌间杀伤系统发挥作用。本研究探讨了T4 SS在土传生防细菌产酶溶杆菌OH 11的杀灭行为中所起的作用。结果表明，L.酶基因OH 11基因组包括编码T4 SS的所有组分和可能参与细菌间杀伤系统的效应子的基因。基因敲除突变体的产生表明，L。enzymogenes OH 11使用T4 SS作为对抗其他土传细菌的主要接触依赖性武器。T4 SS介导的L.酶基因OH 11降低了两种假单胞菌的抗菌和抗真菌活性。但同时也保护了胡萝卜免受胡萝卜腐果胶杆菌的侵染。总的来说，这项研究首次表明T4 SS参与了L.酶基因及其对土壤微生物组中发生的多重相互作用的影响。
Soil microbiome comprises numerous microbial species that continuously interact with each other. Among the modes of diverse interactions, cell-cell killing may play a key role in shaping the microbiome composition. Bacteria deploy various secretion systems to fend off other microorganisms and Type IV Secretion System (T4SS) in pathogenic bacteria was shown to function as a contact-dependent, inter-bacterial killing system only recently. The present study investigated the role played by T4SS in the killing behaviour of the soilborne biocontrol bacterium Lysobacter enzymogenes OH11. Results showed that L. enzymogenes OH11 genome encompasses genes encoding all the components of T4SS and effectors potentially involved in inter-bacterial killing system. Generation of knock-out mutants revealed that L. enzymogenes OH11 uses T4SS as the main contact-dependent weapon against other soilborne bacteria. The T4SS-mediated killing behaviour of L. enzymogenes OH11 decreased the antibacterial and antifungal activity of two Pseudomonas spp. but at the same time, protected carrot from infection by Pectobacterium carotovorum. Overall, this study showed for the first time the involvement of T4SS in the killing behaviour of L. enzymogenes and its impact on the multiple interactions occurring in the soil microbiome.