Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR
Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR
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DOI：
10.1007/s11250-013-0448-6
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发表时间：
2014-01-01
影响因子：
1.7
通讯作者：
Jamal, Syed M.
中科院分区：
文献类型：
--
作者：
Ali, Shahzad;Ali, Qurban;Jamal, Syed M.
布鲁氏菌病在巴基斯坦的牛群中流行。然而，涉及的布鲁氏菌种类和生物群尚不清楚。本研究的目的是从血清阳性的牛奶样本、流产的胎儿以及最近流产的牛和水牛的阴道拭子中分离和鉴定布鲁氏菌。从巴基斯坦波托哈尔高原采集了牛和水牛的血清阳性牛奶样本、流产胎儿和阴道拭子。用改良法瑞尔血清葡萄糖琼脂分离布鲁氏菌。分离株采用常规生物分型方法进行鉴定，分子分型采用属(B4/B5)和种特异性(流产布鲁氏菌、羊布鲁氏菌、猪布鲁氏菌)聚合酶链式反应(PCR)。从牛奶(n=5)、流产胎儿(n=13)和阴道拭子(n=12)中分离到30株。分离株以牛为主(56.7%)。经常规生物分型和种属特异性聚合酶链式反应鉴定，均为流产杆菌生物型1。这项初步研究首次报告了巴基斯坦牛和水牛中流产杆菌生物群1的流行情况。
Brucellosis is endemic in bovines in Pakistan. The Brucella species and biovars involved, however, are unknown. The objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. The seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the Potohar Plateau, Pakistan. Isolation of brucellae was done on modified Farrell's serum dextrose agar. Isolates were characterized by conventional biotyping methods, while molecular typing was done by genus (B4/B5) and species-specific (Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis) polymerase chain reaction (PCR). A total of 30 isolates were recovered from milk (n = 5), aborted fetuses (n = 13), and vaginal swabs (n = 12). Most isolates were from cattle (56.7 %). All of them were identified as B. abortus biovar 1 based on conventional biotyping methods and genus and species-specific PCR. This preliminary study provides the first report on the prevalence of B. abortus biovar 1 in cattle and buffaloes in Pakistan.