Hormonal status of the pollen-pistil system at the progamic phase of fertilization after compatible and incompatible pollination in Petunia hybrida L.
Hormonal status of the pollen-pistil system at the progamic phase of fertilization after compatible and incompatible pollination in Petunia hybrida L.
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DOI：
10.1007/s00497-003-0189-1
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发表时间：
2003-11-01
影响因子：
--
通讯作者：
Zakharova, E
中科院分区：
文献类型：
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作者：
Kovaleva, L;Zakharova, E
矮牵牛花粉-雌蕊系统的激素状况。在受精的排卵期进行了研究。测定了亲和授粉和自交不亲和授粉后8 h雌蕊及其部位（柱头、花柱和子房）中吲哚乙酸（IAA）、脱落酸（阿坝）和细胞分裂素的含量以及乙烯释放速率。在这两个授粉，植物激素存在于不同的比例在柱头，花柱和子房：柱头是乙烯合成的主要网站，并包含90%的阿坝，而风格包含80%的总细胞分裂素含量在授粉雌蕊。子房中激素水平相对较低并不影响花粉-雌蕊系统的激素状态。雄配子体与柱头组织的相互作用使花粉-雌蕊系统的乙烯释放量增加7 ~ 10倍，IAA含量增加1.5 ~ 2.0倍。花粉管生长自交不亲和授粉后，在兼容授粉，相反，伴随着3倍的阿坝含量增加的柱头和花柱和5倍高的细胞分裂素含量在花柱组织。因此，柱头的乙烯/阿坝状态可能在控制授粉过程中花粉粒的粘附、水合和萌发过程中发挥作用，而花柱的生长素/细胞分裂素状态可能参与控制花粉管的生长。
The hormonal status of the pollen-pistil system in Petunia hybrida L. during the progamic phase of fertilization was investigated. The contents of indolyl-3-acetic acid (IAA), abscisic acid (ABA), and cytokinins, as well as the rate of ethylene production in the pistils and their parts (stigma, style, and ovary) were measured over an 8-h period following compatible and self-incompatible pollination. In both pollinations, the phytohormones were present in various proportions in the stigma, style and ovary: the stigma was the main site of ethylene synthesis and contained 90% of the ABA, while the style contained 80% of the total cytokinin content in the pollinated pistil. Relatively low levels of hormones in the ovary did not influence the hormonal status of the pollen-pistil system. The interaction of the male gametophyte with the stigmatic tissues was accompanied by a 7- to 10-fold increase in ethylene production and a 1.5- to 2.0-fold increase in IAA content in the pollen-pistil system over 0-4 h. Pollen tube growth after self-incompatible pollination, in contrast to compatible pollination, was accompanied by a 3-fold increase in the ABA content in the stigma and style and by a 5-fold higher cytokinin content in the stylar tissues. Thus, the ethylene/ABA status of the stigma may play a role in controlling the processes of adhesion, hydration, and germination of pollen grains during pollination while the auxin/cytokinin status of the style may be involved in controlling pollen tube growth.