Mimicking of Human Body Electrical Characteristic for Easier Translation of Plasma Biomedical Studies to Clinical Applications
Mimicking of Human Body Electrical Characteristic for Easier Translation of Plasma Biomedical Studies to Clinical Applications
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DOI：
10.1109/trpms.2019.2936667
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发表时间：
2020-05-01
影响因子：
4.4
通讯作者：
Robert, E.
中科院分区：
文献类型：
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作者：
Stancampiano, A.;Chung, T-H;Robert, E.
非热等离子体（NTP）的医疗应用现在已经很好地建立，但从体外等离子体效应到体内效应的转换仍然远远不够直观。在各种可能的原因中，平移可能受到对不同目标的电特性的松散控制的干扰（例如，细胞培养皿、动物模型），这是一个通常被忽略的参数，在论文中也经常没有提到。本文的目的是提高人们对如何靶向电参数（例如，电导率、电势$\dots $）在确定等离子体处理条件中起主要作用。这种效应在血浆医学中特别相关，我们从治疗小的体外样本转移到人类，通过动物模型。通过电学测量、光学发射光谱和基本液体分析，比较了具有不同电学特性的靶上的等离子体条件。通常测试的体外靶标诱导产生的血浆与接触人体产生的血浆显著不同。我们展示了如何通过一个基本的和易于实现的电路，它是可能的“补偿”在体外模型，小鼠和人体之间的电差异，并在这样一种方式，以达到在体外和体内目标之间的更可重复的治疗条件。所提出的用于控制目标电参数的方法可以极大地有利于从体外和体内模型到患者的过渡，用于为生物医学应用开发的许多等离子体装置。
Nonthermal plasma (NTP) medical applications are now well established but the translation from in vitro plasma effects to in vivo effects remains far from being intuitive. Among various possible reasons, the translation may be disturbed by a loose control over the electrical characteristics of the different targets (e.g., cell culture dish, animal models) met during the development process, a parameter generally neglected and often unmentioned in papers. The aim of this article is to raise consciousness on how target electrical parameters (e.g., conductivity, electric potential $\dots $ ) play a major role in determining plasma treatment conditions. This effect is of particular relevance in plasma medicine where we move from treating small in vitro samples to humans, passing by animal models. The plasma conditions on targets with different electrical characteristics are compared by means of electrical measurements, optical emission spectroscopy, and basic liquid analysis. Commonly tested in vitro targets induce the generation of a plasma significantly different from that produced in contact with a human body. We demonstrate how by means of a basic and easy to implement electrical circuit it is possible to "compensate" the electrical differences between in vitro models, mice and human body and in such a way to reach more reproducible treatment conditions between in vitro and in vivo targets. The proposed method for the control of the target electrical parameters could greatly favor the transition from in vitro and in vivo models to patients for many plasma devices developed for biomedical applications.