Protocol to measure bacterial intake and gut clearance of Caenorhabditis elegans.
Protocol to measure bacterial intake and gut clearance of Caenorhabditis elegans.
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DOI：
10.1016/j.xpro.2022.101558
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发表时间：
2022-09-16
期刊：
影响因子：
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通讯作者：
Sun, Jingru
中科院分区：
文献类型：
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作者：
Wibisono, Phillip;Sun, Jingru
线虫秀丽隐杆线虫是免疫和宿主-病原体相互作用研究的常见模型生物。在本协议中，我们描述了测量暴露于致病或非致病细菌的成年线虫的咽部泵送和排便率的技术。量化病原体感染期间排便率的变化可以解释可能影响生存的行为变化。我们还详细介绍了如何同步线虫并将同步动物暴露于致病或非致病细菌。有关该协议的使用和执行的完整详细信息，请参阅。测量线虫进食和排便率的变化 将同步线虫暴露于病原菌的方案 进食和排便率分析 出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和道德指南。线虫秀丽隐杆线虫是免疫和宿主-病原体相互作用研究的常见模型生物。在本协议中，我们描述了测量暴露于致病或非致病细菌的成年线虫的咽部泵送和排便率的技术。量化病原体感染期间排便率的变化可以解释可能影响生存的行为变化。我们还详细介绍了如何同步线虫并将同步动物暴露于致病或非致病细菌。
The nematode Caenorhabditis elegans is a common model organism for studies in immunity and host-pathogen interactions. In this protocol, we describe techniques for measuring pharyngeal pumping and defecation rates of adult C. elegans exposed to either pathogenic or non-pathogenic bacteria. Quantifying changes in pumping or defecation rates during pathogenic infection can account for behavioral changes that may affect survival. We also detail how to synchronize C. elegans and expose the synchronize animals to either pathogenic or non-pathogenic bacteria. For complete details on the use and execution of this protocol, please refer to. Measuring changes in feeding and defecation rates in C. elegans Protocol for exposing synchronized C. elegans to pathogenic bacteria Analysis of feeding and defecation rates Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The nematode Caenorhabditis elegans is a common model organism for studies in immunity and host-pathogen interactions. In this protocol, we describe techniques for measuring pharyngeal pumping and defecation rates of adult C. elegans exposed to either pathogenic or non-pathogenic bacteria. Quantifying changes in pumping or defecation rates during pathogenic infection can account for behavioral changes that may affect survival. We also detail how to synchronize C. elegans and expose the synchronize animals to either pathogenic or non-pathogenic bacteria.