A Pulsed Electromagnetic Field Protects against Glutamate-Induced Excitotoxicity by Modulating the Endocannabinoid System in HT22 Cells.
A Pulsed Electromagnetic Field Protects against Glutamate-Induced Excitotoxicity by Modulating the Endocannabinoid System in HT22 Cells.
复制标题
脉冲电磁场通过调节 HT22 细胞中的内源性大麻素系统来防止谷氨酸诱导的兴奋性毒性。
DOI：
10.3389/fnins.2017.00042
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发表时间：
2017
影响因子：
4.3
通讯作者：
Xu Q
中科院分区：
文献类型：
--
作者：
Li X;Xu H;Lei T;Yang Y;Jing D;Dai S;Luo P;Xu Q
谷氨酸诱导的兴奋性毒性在许多神经系统疾病的发病机制中是常见的。脉冲电磁场（PEMF）对神经系统具有治疗作用，但其与兴奋性毒性相关的具体机制尚不清楚。我们研究了PEMF暴露在调节谷氨酸诱导的兴奋性毒性通过内源性大麻素（eCB）系统的作用。PEMF暴露改善HT 22细胞兴奋性毒性后的生存能力，并减少乳酸脱氢酶的释放和细胞死亡。一种eCB受体1（CB 1 R）特异性抑制剂抑制了PEMF暴露的保护作用，即使没有观察到CB 1 R表达的变化。PEMF暴露后N-花生四烯酸乙醇酰胺（AEA）和2-花生四烯酸甘油（2-AG）的升高表明PEMF的神经保护作用与调节eCB代谢系统有关。CB 1 R/ERK信号通路是PEMF调节兴奋性毒性的重要下游通路。这些结果表明，PEMF暴露通过促进eCB/CB 1 R/ERK信号通路导致对兴奋性毒性的神经保护作用。因此，脉冲电磁场可能成为一种潜在的预防和治疗神经系统疾病的物理治疗技术。
Glutamate-induced excitotoxicity is common in the pathogenesis of many neurological diseases. A pulsed electromagnetic field (PEMF) exerts therapeutic effects on the nervous system, but its specific mechanism associated with excitotoxicity is still unknown. We investigated the role of PEMF exposure in regulating glutamate-induced excitotoxicity through the endocannabinoid (eCB) system. PEMF exposure improved viability of HT22 cells after excitotoxicity and reduced lactate dehydrogenase release and cell death. An eCB receptor 1 (CB1R) specific inhibitor suppressed the protective effects of PEMF exposure, even though changes in CB1R expression were not observed. Elevation of N-arachidonylethanolamide (AEA) and 2-arachidonylglycerol (2-AG) following PEMF exposure indicated that the neuroprotective effects of PEMF were related to modulation of the eCB metabolic system. Furthermore, CB1R/ERK signaling was shown to be an important downstream pathway of PEMF in regulating excitotoxicity. These results suggest that PEMF exposure leads to neuroprotective effects against excitotoxicity by facilitating the eCB/CB1R/ERK signaling pathway. Therefore, PEMF may be a potential physical therapeutic technique for preventing and treating neurological diseases.