EFFECTS OF INTRACEREBROVENTRICULAR INJECTION OF NEUROPEPTIDE-Y ON ENERGY-METABOLISM
EFFECTS OF INTRACEREBROVENTRICULAR INJECTION OF NEUROPEPTIDE-Y ON ENERGY-METABOLISM
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DOI：
10.1152/ajpregu.1991.260.2.r321
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发表时间：
1991-02-01
影响因子：
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通讯作者：
LEVINE, AS
中科院分区：
文献类型：
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作者：
BILLINGTON, CJ;BRIGGS, JE;LEVINE, AS
我们的目的是了解中枢注射神经肽Y（NPY）是否会改变棕色脂肪产热和白色脂肪脂蛋白脂酶活性。 以下三组Sprague-Dawley大鼠在24小时内接受5次注射进入右侧脑室：1）NPY（5 μ g/注射）和随意食物; 2）NPY（5 μ g/注射）和限制控制摄入的食物; 3）盐水注射和随意食物。 NPY自由进食组比盐水对照组或NPY食物限制动物消耗更多的食物。 棕色脂肪产热活性，GDP结合评估，减少相对于盐水对照在两个NPY治疗组。 与生理盐水对照组相比，两个NPY治疗组的白色脂肪脂蛋白脂酶活性显著增加。 NPY对棕色和白色脂肪的影响不能通过测量血清胰岛素、胰高血糖素、葡萄糖或其他代谢产物来解释。 在后续实验中，我们询问食物是否是表达NPY效应所必需的。 棕色脂肪线粒体GDP结合表明NPY的影响，即使没有食物摄入。 我们的结论是，脑室内注射神经肽Y促进白色脂肪的脂质储存，并减少棕色脂肪产热，除了其已知的刺激食物摄入量的影响。
Our objective was to find out if central injection of neuropeptide Y (NPY) would alter brown fat thermogenesis and white fat lipoprotein lipase activity. The following three groups of Sprague-Dawley rats received five injections over 24 h into the right lateral ventricle: 1) NPY (5 mu-g/injection) and ad libitum food; 2) NPY (5 mu-g/injection) and food restricted to control intake; 3) saline injection and ad libitum food. The NPY ad libitum-fed group consumed more food than the saline controls or NPY food-restricted animals. Brown fat thermogenic activity, assessed by GDP binding, was decreased relative to saline controls in both NPY-treated groups. White fat lipoprotein lipase activity was greatly increased in both NPY treatment groups compared with saline controls. The NPY effects on brown and white fat were not explained by measures of serum insulin, glucagon, glucose, or other metabolites. In a follow-up experiment, we asked whether food was necessary for expression of the NPY effects. Brown fat mitochondrial GDP binding indicated NPY effect even when no food was ingested. We conclude that intracerebroventricular administration of NPY promotes white fat lipid storage and decreases brown fat thermogenesis in addition to its known effect of stimulating food intake.