P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line
P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line
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DOI：
10.3892/ijo.2010.862
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发表时间：
2011-02-01
影响因子：
5.2
通讯作者：
Lalloue, Fabrice
中科院分区：
文献类型：
--
作者：
Giraud, Stephanie;Loum, Elodie;Lalloue, Fabrice
P75神经营养素受体(p75(NTR))是一种细胞表面受体，可诱导多种细胞类型的细胞凋亡。这种受体在中枢神经系统的发育中起着重要作用，在一些成年脑细胞中也有表达。P75(NTR)在细胞凋亡或存活中的作用可能在细胞内稳态中起重要作用，因此p75(NTR)可能与肿瘤抵抗死亡有关。在这项研究中，我们研究了p75(NTR)在人脑胶质母细胞瘤细胞系中的表达。免疫荧光显微镜检测高尔基体中p75(NTR)受体，或在高尔基体摘除后检测到p75(NTR)受体，可能与细胞凋亡减少导致细胞癌变有关。这一假说得到了配体在该细胞系中诱导的细胞凋亡缺失的支持。我们的观察表明，p75(NTR)可以被隔离在高尔基复合体中，然后可能在一定程度上与细胞对凋亡的抵抗和脑瘤的形成有关。
The P75 neurotrophin receptor (p75(NTR)) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75(NTR) could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75(NTR) in a human glioblastoma cell line. Detection of p75(NTR) receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. Our observations show that p75(NTR) can be sequestered in the Golgi complex and could then be, in part, responsible for the cell resistance to apoptosis and for brain tumor formation.