Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos
Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos
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DOI：
10.1038/nprot.2012.120
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发表时间：
2012-10
期刊：
影响因子：
14.8
通讯作者：
Martin Zeeb;Jennifer Axnick;L. Planas-Paz;Thorsten Hartmann;Boris Strilic;E. Lammert
中科院分区：
文献类型：
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作者：
Martin Zeeb;Jennifer Axnick;L. Planas-Paz;Thorsten Hartmann;Boris Strilic;E. Lammert
新生血管和淋巴管的形成涉及许多生理和病理过程，包括器官和肿瘤的生长、癌细胞转移、液体引流和淋巴水肿。因此，在哺乳动物系统中操纵血管形成的能力是研究人员特别感兴趣的。在这里，我们描述了一种在体内培养的小鼠胚胎中对新生血管和萌发的血液和淋巴管发育进行药理学处理的方法。所描述的方案还可用于评估正在生长的哺乳动物组织中的药理制剂的性质，以及操纵其他发育过程。从胚胎分离到图像量化的整个过程需要3-5天，这取决于胚胎的分析和年龄。
Formation of new blood and lymphatic vessels is involved in many physiological and pathological processes, including organ and tumor growth, cancer cell metastasis, fluid drainage and lymphedema. Therefore, the ability to manipulate vascularization in a mammalian system is of particular interest to researchers. Here we describe a method for pharmacological manipulation ofde novoand sprouting blood and lymphatic vascular development inex vivo–cultured mouse embryos. The described protocol can also be used to evaluate the properties of pharmacological agents in growing mammalian tissues and to manipulate other developmental processes. The whole procedure, from embryo isolation to image quantification, takes 3–5 d, depending on the analysis and age of the embryos.