Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways
Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways
复制标题
DOI：
10.1039/c6fo01873d
复制
发表时间：
2017-03-01
期刊：
影响因子：
6.1
通讯作者：
Han, Jianchun
中科院分区：
文献类型：
--
作者：
Jiang, Fan;Guan, Haining;Han, Jianchun
沙棘长期以来被用作调节胆固醇、缓解心绞痛和消炎的功能性食品。黄酮类化合物是沙棘的主要活性成分之一。我们研究了沙棘类黄酮（SF）处理对介导炎症的两条途径，即丝裂原活化蛋白激酶（MAPK）和核因子-κ B（NF-β B）途径的影响，以探讨SF在脂多糖（LPS）刺激的RAW 264. 7巨噬细胞中的抗炎活性。SF通过调节诱导型一氧化氮合酶（iNOS）和环氧合酶-2（考克斯-2）基因的作用抑制LPS诱导的一氧化氮（NO）和前列腺素E2（PGE（2））的过度产生。此外，SF下调促炎细胞因子的产生和mRNA表达，包括肿瘤坏死因子（TNF）-α，白细胞介素（IL）-6和IL-1 β。此外，SFs还能抑制p38和应激活化蛋白激酶/JNK（stress-activated protein kinase/jun aminotomal kinase，SAPK/JNK）MAPK通路的磷酸化，并通过阻断NF-κ B α抑制蛋白（I kappa B-alpha）的磷酸化和降解，减少NF-κ B的核转位，阻止NF-κ B的活化。基于这些发现，SF可能通过MAPK和NF-κ B途径调节炎症介质的释放来发挥其对炎症的抑制作用。SF强调了使用具有抗炎作用的功能性食品对抗炎症性疾病的潜在益处。
Sea buckthorn has long been used as a functional food to regulate cholesterol, relieve angina, and diminish inflammation. Flavonoids are one of the main active components in sea buckthorn. We investigated the effects of sea buckthorn flavonoid (SF) treatment on two pathways that mediate inflammation, the mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-beta B) pathways, to explore the anti-inflammatory activity of SFs in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The LPS-induced over-production of nitric oxide (NO) and prostaglandin E2 (PGE(2)) was inhibited by SFs through a mechanism related to the modulatory effects of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) genes. Additionally, SFs downregulated the production and mRNA expression of pro-inflammatory cytokines including tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-1 beta. Moreover, SFs inhibited the phosphorylation of the p38 and stress-activated protein kinase/jun aminoterminal kinase (SAPK/JNK) MAPK pathways, and they reduced the nuclear translocation of NF-kappa B to prevent its activation by blocking the phosphorylation and degradation of inhibitor protein of NF-kappa B a (I kappa B-alpha). Based on these findings, SFs may exert their inhibitory effects on inflammation by regulating the release of inflammatory mediators through the MAPK and NF-kappa B pathways. SFs highlight the potential benefits of using functional foods with anti-inflammatory actions to combat inflammatory diseases.