SDE3 encodes an RNA helicase required for posttranscriptional gene silencing in Arabidopsis
SDE3 encodes an RNA helicase required for posttranscriptional gene silencing in Arabidopsis
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DOI：
10.1093/emboj/20.8.2069
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发表时间：
2001-04-17
期刊：
影响因子：
11.4
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
--
作者：
Dalmay, T;Horsefield, R;Baulcombe, DC
转录后基因沉默（PTGS）为植物提供抗病毒感染的保护，并可以抑制转基因的表达，携带ADE 3基因座突变的拟南芥植物在由绿色荧光蛋白转基因介导的PTGS中存在缺陷。SDE 3与SDE 1编码的RNA聚合酶一样，作用于TRV介导的PTGS的机制中的一个阶段，SDE 3的产物类似于RNA解旋酶样蛋白，包括小鼠中的GB 110和果蝇和人类中的其他蛋白。这些蛋白质与Upf 1 p和SMG-2相似，但明显不同，它们是酵母和秀丽隐杆线虫中无义介导的mRNA衰变所需的，在SMG-2的情况下，是PTGS所需的。
post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes, Arabidopsis plants carrying mutations at the SDE3 locus are defective in PTGS mediated by a green fluorescent protein transgene. However, PTGS mediated by tobacco rattle virus (TRV) was not affected by sde3, From these results we conclude that SDE3, like the previously described RNA polymerase encoded by SDE1, acts at a stage in the mechanism that is circumvented when PTGS is mediated by TRV, The product of SDE3 is similar to RNA helicase-like proteins including GB110 in mouse and other proteins in Drosophila and humans. These proteins are similar to, but clearly distinct from Upf1p and SMG-2, which are required for nonsense-mediated mRNA decay in yeast and Caenorhabditis elegans and, in the case of SMG-2, for PTGS.