Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens
Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens
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用井冈霉素抑制海藻糖酶活性会破坏褐飞虱的海藻糖和几丁质生物合成途径
DOI：
10.1016/j.pestbp.2016.10.003
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发表时间：
2017-04-01
影响因子：
4.7
通讯作者：
Wang, Shigui
中科院分区：
文献类型：
--
作者：
Tang, Bin;Yang, Mengmeng;Wang, Shigui
海藻糖酶（Trehalase， TRE）是海藻糖降解的关键酶，在昆虫生长和几丁质合成中具有重要作用。虽然validamycin具有通过抑制TRE活性来控制害虫的潜力，但尚不清楚validamycin是否同时作用于海藻糖和几丁质的代谢。与对照组相比，注射不同剂量的缬霉素溶液后，TRE1、TRE2活性和葡萄糖、糖原含量均显著降低，海藻糖含量显著升高。结果表明，注射不同浓度的药液48 h后，13 ~ 38%的昆虫出现表型异常，10 ~ 57%的昆虫死亡；几丁质含量也显著降低。验证霉素通过抑制两种TRE5的活性改变海藻糖、糖原和几丁质代谢相关基因的相对表达水平。结果表明，3个TRE和2个海藻糖-6-磷酸合成酶（TPS）基因的表达水平升高，而GP；CHS1及其两个转录本CHS1a、CHS1b；6种几丁质酶，包括Cht3、Cht4、Cht5、Cht6、Cht7、Cht9；注射任意浓度的有效性霉素48 h后，HK、G6PI2、GFAT、GNPNA、PAGM1、UAP、WL、CI、AP基因均较对照组显著降低。这些结果表明，有效性霉素通过抑制两种TREs的活性，改变了N. lugens几丁质的合成和降解，影响海藻糖和几丁质代谢相关基因的表达。TRE抑制剂的开发可能在未来提供有效的害虫防治。(C) 2016 Elsevier Inc .版权所有
Trehalase (TRE) is a key enzyme in trehalose degradation and has important functions in insect growth and chitin synthesis. Though validamycin has the potential for pest control by suppressing TRE activities, it is not known whether validamycin acts on both trehalose and chitin metabolism. TRE1 and TRE2 activities and glucose and glycogen contents decreased significantly after the injection of different doses of validamycin solution compared with the control group, while the trehalose content increased significantly. Overall, it showed that about 13 to 38% insects was appeared abnormal phenotypes, and 10 to 57% of insects died 48 h after injection of solutions with different concentrations of validamycin; the chitin content also decreased significantly. Validamycin altered the relative expression levels of trehalose, glycogen and chitin metabolism-related genes by suppressing the activities of two TRE5. We showed that the expression levels of three TRE and two trehalose-6-phosphate synthase (TPS) genes increased, while the expression levels of GP; CHS1 and its two transcripts, CHS1a, CHS1b; six chitinases, including Cht3, Cht4, Cht5, Cht6, Cht7, Cht9; and the HK, G6PI2, GFAT, GNPNA, PAGM1, UAP, WL, CI and AP genes decreased significantly 48 h after the injection of any validamycin concentration compared with the control group. These results demonstrate that by inhibiting the activities of two TREs, validamycin alters N. lugens chitin synthesis and degradation and affects trehalose and chitin metabolism-related gene expression. The development of TRE inhibitors may provide effective pest control in the future. (C) 2016 Elsevier Inc All rights reserved.