Prognostic impact of nestin in resected large cell neuroendocrine carcinoma of the lung.
Prognostic impact of nestin in resected large cell neuroendocrine carcinoma of the lung.
复制标题
巢蛋白对切除的肺大细胞神经内分泌癌的预后影响。
DOI：
10.1016/j.lungcan.2012.03.025
复制
发表时间：
2012
期刊：
影响因子：
5.3
通讯作者：
Masuda N.
中科院分区：
文献类型：
--
作者：
Ryuge S;Sato Y;Jiang Shi-X;Wang G;Matsumoto T;Katone K;Inoue H;Iyoda A;Satoh Y;Yoshimura H;Masuda N.
背景肺大细胞神经内分泌癌（LCNEC）是一种高度恶性的神经内分泌癌，具有侵袭性的临床表现。巢蛋白是一种VI类中间丝蛋白，在中枢神经系统发育过程中在干/祖细胞中表达。最近，我们报道了巢蛋白表达是NSCLC切除患者生存率较差的预后指标。在本研究中，我们的目的是确定其预后意义与切除LCNEC.MATERIALS和METHODSNESTIN表达的患者的生存在30例切除LCNECs的肿瘤细胞进行了免疫组化研究，其与临床病理参数，包括Ki-67标记指数（LI）和TTF-1的表达进行了评估。Kaplan-Meier生存分析和考克斯比例风险模型分析nestin表达对生存的影响。在临床病理学上，虽然nestin表达与年龄、性别、吸烟习惯、p-TNM分期、肿瘤大小、淋巴结状态或TTF-1表达之间没有显著相关性，但nestin表达与高Ki-67 LI显著相关（P=0.012）。在生存分析中，巢蛋白表达与LCNEC患者的预后不良显著相关（P=0.016）。考克斯比例回归模型证实nestin表达的粗风险比（95%CI）为3.40（1.18-9.77）。结论本研究表明nestin表达似乎是切除LCNEC患者生存率较低的预后指标，但其预后意义仍需在更大的患者群体中证实。
BACKGROUNDLarge cell neuroendocrine carcinoma (LCNEC) of the lung is categorized as a high-grade neuroendocrine carcinoma with an aggressive clinical behavior. Nestin is a class VI intermediate filament protein expressed in stem/progenitor cells during central nervous system development. Recently, we reported that nestin expression is a prognostic indicator of a poorer survival probability in patients with resected NSCLC. In the present study, we aimed to determine its prognostic significance concerning survival in patients with resected LCNEC.MATERIALS AND METHODSNestin expression in tumor cells was immunohistochemically studied in 30 patients with resected LCNEC, and its associations with clinicopathologic parameters including the Ki-67 labeling index (LI) and TTF-1 expression were evaluated. Kaplan–Meier survival analysis and Cox proportional hazards models were used to estimate the effect of nestin expression on survival.RESULTSNestin expression was observed in 8 of the 30 (26.7%) LCNECs. Clinicopathologically, although no significant association between nestin expression and age, gender, smoking habits, p-TNM stage, tumor size, nodal status, or TTF-1 expression was observed, nestin expression was significantly associated with a high Ki-67 LI (P=0.012). On survival analysis, nestin expression was significantly associated with a poorer prognosis in patients with LCNEC (P=0.016). The Cox proportional regression model confirmed that the crude hazard ratio (95%CI) of nestin expression was 3.40 (1.18–9.77).CONCLUSIONSThe present study suggests that nestin expression seems to be a prognostic indicator of a poorer survival probability in patients with resected LCNEC, although its prognostic significance still requires confirmation with larger patient populations.