Analysis of 8-hydroxydeoxyguanosine among workers exposed to diesel particulate exhaust: Comparison with urinary metabolites and PAH air monitoring
Analysis of 8-hydroxydeoxyguanosine among workers exposed to diesel particulate exhaust: Comparison with urinary metabolites and PAH air monitoring
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DOI：
10.1080/10715760500190115
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发表时间：
2005-09-01
影响因子：
3.3
通讯作者：
Savela, K
中科院分区：
文献类型：
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作者：
Harri, M;Svoboda, P;Savela, K
用尿液和DNA样品中8-羟基-2‘-脱氧鸟苷(8-OHdG)测定的DNA氧化损伤和修复，与汽车修理厂和垃圾收集工人的工作相关柴油尾气接触进行了研究。测定了29名作业工人和36名对照组工人班前和班后尿8-OHdG水平的季节变化。接触组工人班后8-OHdG(单位分子质量分数)的+/-SE平均值冬季为1.52+/-0.44，夏季为1.61+/-0.33；对照组冬季为1.56+/-0.61，夏季为1.43+/-0.49。冬季(p=0.923)和夏季(p=0.350)接触者尿8-OHdG水平与对照组无显著差异。经工作年限、年龄、前吸烟/不吸烟和体重指数调整后的线性混合模型表明，在同一工人身上分析的8-OHdG和15个多环芳烃空气浓度之间以及8-OHdG和7个多环芳烃单羟基代谢物之间没有显著的剂量暴露关系。19名接触者和18名对照组的单个核细胞DNA中也检测了8-OHdG。8-OHdG/未修饰的2‘-脱氧鸟苷(8-OHdG/105dG-SE)平均值为4.89+/-0.17，对照组为4.11+/-0.16，与尿8-OHdG水平无相关性(r=0.01，n=28，P=0.96)。工作场所暴露的多环芳烃主要由挥发性化合物组成，特别是萘，这表明通过呼吸道接触的多环芳烃较少，多环芳烃对ROS的诱导作用也很小。
Oxidative DNA damage and repair, as measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine and DNA samples were studied in association with work-related diesel exhaust exposure among garage and waste collection workers. Seasonal variations of the urinary 8-OHdG levels in pre- and two post-workshift urine samples of 29 exposed workers and 36 control persons were evaluated. The mean +/- SE levels of post-workshift 8-OHdG (mu mol/mol crea) were 1.52 +/- 0.44 in winter and 1.61 +/- 0.33 in summer for the exposed workers, and 1.56 +/- 0.61 in winter and 1.43 +/- 0.49 in summer for the controls, respectively. No significant difference in the urinary 8-OHdG levels between exposed workers and control subjects in winter (p = 0.923) and summer (p = 0.350) was observed. A linear mixed model, adjusted for years of employment, age, ex/nonsmoking and BMI, indicated no significant dose exposure-relationships between the urinary 8-OHdG and 15 PAH air concentrations nor between the 8-OHdG and 7 PAH monohydroxy-metabolites analyzed in the same workers. 8-OHdG was also analyzed in the mononuclear cell DNA of 19 exposed and 18 control subjects. The mean value of 8-OHdG/non-modified 2'-deoxyguanosine (8-OHdG/105 dG - SE) were 4.89 +/- 0.17 for the exposed and 4.11 +/- 0.16 for the control persons, which showed no correlation with the urinary 8-OHdG levels (r= 0.01, n = 28, P= 0.96). The PAH exposure at workplaces was mainly composed of volatile compounds, particularly naphthalene, suggesting low exposure through the respiratory tract and a low effect of PAH in ROS induction.