Increased expressions of claudin 4 and 7 in atypical adenomatous hyperplasia and adenocarcinoma of the lung
Increased expressions of claudin 4 and 7 in atypical adenomatous hyperplasia and adenocarcinoma of the lung
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DOI：
10.1007/s00795-016-0135-6
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发表时间：
2016-02
影响因子：
1.8
通讯作者：
G. Yamada;M. Murata;A. Takasawa;M. Nojima;Yuki Mori;N. Sawada;Hiroki Takahashi
中科院分区：
文献类型：
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作者：
G. Yamada;M. Murata;A. Takasawa;M. Nojima;Yuki Mori;N. Sawada;Hiroki Takahashi
紧密连接蛋白（claudin，Cldn）是紧密连接的主要组成部分，其异常表达可能在肿瘤发生中起重要作用。为了阐明肺腺癌癌变过程中紧密连接的异常，我们检测了人肺切除材料中Cldn 4和Cldn 7的免疫组化表达。研究了86例患者的肺切除标本，其中非典型腺瘤性增生（AAH）16例，原位腺癌（AIS）19例，浸润性腺癌（ADC）32例，AIS合并AAH 5例，ADC合并AAH 2例，ADC合并AIS 10例，ADC合并AIS和AAH 2例。免疫组织化学染色（IHC）评分定义为染色的程度和强度。AIS和ADC中Cldn 4的IHC评分显著高于肺泡上皮（AE）和AAH（两者均为p< 0.001）。此外，AAH评分显著高于AE（p< 0.001）。ADC中的Cldn 7评分与AE和AAH相比显著增加（两者均为p< 0.001）。这些结果表明，Cldn 4-表达的增加可能参与腺癌发生过程中的早期分子事件，而Cldn 7-表达的增加可能与肿瘤的侵袭或进展有关。
Abnormal expression of claudin (Cldn), the main constituent of tight junctions, may play a crucial role in carcinogenesis. To elucidate these abnormalities of tight junctions in lung adenocarcinoma during carcinogenesis, we examined immunohistochemical expressions of Cldn4 and Cldn7 in human lung resection materials. Lung resection specimens from 86 patients were studied, including 16 atypical adenomatous hyperplasia (AAH), 19 adenocarcinoma in situ (AIS), 32 invasive adenocarcinoma (ADC), 5 AIS with AAH, 2 ADC with AAH, 10 ADC with AIS, and 2 ADC with AIS and AAH. The immunohistochemical staining (IHC) score was defined for both the extent and intensity of staining. IHC score for Cldn4 in AIS and ADC was significantly higher than that in alveolar epithelium (AE) and AAH (p< 0.001 for both). In addition, the AAH score was significantly higher than that in AE (p< 0.001). The Cldn7 score in ADC was significantly increased compared with AE and AAH (p< 0.001 for both). These results suggested that increase of Cldn4-expression may be involved in early molecular events during carcinogenesis of adenocarcinoma, whereas increase of Cldn7-expression may be associated with tumor invasion or progression.