Effects of Light Isoflurane Anesthesia on Organization of Direction and Orientation Selectivity in the Superficial Layer of the Mouse Superior Colliculus
Effects of Light Isoflurane Anesthesia on Organization of Direction and Orientation Selectivity in the Superficial Layer of the Mouse Superior Colliculus
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DOI：
10.1523/jneurosci.1196-21.2021
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发表时间：
2022-01-26
影响因子：
5.3
通讯作者：
Isa, Tadashi
中科院分区：
文献类型：
--
作者：
Kasai, Masatoshi;Isa, Tadashi
上级丘（SC）是整合视觉和多通道感觉信息的中脑中心。SC中的神经元表现出方向和取向选择性。最近的研究报道，具有相似偏好的神经元在小鼠SC中形成簇（Ahmadlou和Heimel，2015; Feinberg和Meister，2015; de Malmazet等人，2018年; Li等人，2020年）。然而，关于这些集群如何在SC内组织仍然存在争议（Inayat等人，2015年; Chen等人，2021年）。在这里，我们发现不同的大脑状态（即，清醒或用异氟烷麻醉）改变了雄性和雌性小鼠中单个SC神经元和神经元群体组织的选择性。使用双光子钙21成像，我们研究了单个神经元的反应和他们的群体反应的空间模式。异氟烷麻醉下，方向选择性增加，观察到大量的方向选择性细胞时，与清醒状态相比，而方向选择性细胞的比例在两种条件下是相似的。此外，方向和方向选择性细胞位于较近的位置表现出更相似的偏好，集群样的空间格局得到加强。异氟醚麻醉下方向选择性神经元的抑制反应也减少。因此，空间组织的反应模式的变化被认为是因为在局部电路中的兴奋和抑制的平衡的变化，兴奋占主导地位。这些结果提供了新的见解的可能性，功能组织的功能选择性在大脑中的大脑状态的影响。
The superior colliculus (SC) is the midbrain center for integrating visual and multimodal sensory information. Neurons in the SC exhibit direction and orientation selectivity. Recent studies reported that neurons with similar preferences formed clusters in the mouse SC (Ahmadlou and Heimel, 2015; Feinberg and Meister, 2015; de Malmazet et al., 2018; Li et al., 2020). However, it remains controversial as to how these clusters are organized within the SC (Inayat et al., 2015; Chen et al., 2021). Here, we found that different brain states (i.e., awake or anesthetized with isoflurane) changed the selectivity of individual SC neurons and organizations of the neuronal population in both male and female mice. Using two-photon Ca21 imaging, we examined both individual neuronal responses and the spatial patterns of their population responses. Under isoflurane anesthesia, orientation selectivity increased and a larger number of orientation-selective cells were observed when compared with the awake condition, whereas the proportions of direction-selective cells were similar in both conditions. Furthermore, direction- and orientation-selective cells located at closer positions showed more similar preferences, and cluster-like spatial patterns were enhanced. Inhibitory responses of direction-selective neurons were also reduced under isoflurane anesthesia. Thus, the changes in the spatial organization of response patterns were considered to be because of changes in the balance of excitation and inhibition, with excitation dominance, in the local circuits. These results provide new insights into the possibility that the functional organization of feature selectivity in the brain is affected by brain state.