Arabidopsis formin AtFH6 is a plasma membrane-associated protein upregulated in giant cells induced by parasitic nematodes
Arabidopsis formin AtFH6 is a plasma membrane-associated protein upregulated in giant cells induced by parasitic nematodes
复制标题
DOI：
10.1105/tpc.104.024372
复制
发表时间：
2004-09-01
期刊：
影响因子：
11.6
通讯作者：
Abad, P
中科院分区：
文献类型：
--
作者：
Favery, B;Chelysheva, LA;Abad, P
植物寄生线虫Meloidogyne spp诱导了一个复杂的永久性取食场所，其特征是根细胞再分化为多核肥大巨细胞。我们通过启动子陷阱策略分离出拟南芥双胍基因AtFH6，该基因在巨细胞形成过程中上调。Formins是肌动蛋白成核蛋白，刺激肌动蛋白细丝的重新聚合。我们在这里发现三种1型形成蛋白在巨细胞中上调，并且AtFH6蛋白锚定在质膜上并均匀分布。抑制酿酒酵母bni1Delta bnr1Delta突变体的出芽缺陷表明，AtFH6通过控制肌动蛋白电缆的组装来调节极化生长。我们的研究结果表明，AtFH6可能通过重组肌动蛋白细胞骨架参与了肥厚摄食细胞的各向同性生长。肌动蛋白电缆将作为广泛的质膜和细胞壁生物发生所需的囊泡运输的轨道。因此，确定植物寄生线虫如何将根细胞修饰为巨细胞是鉴定调节细胞生长和形态发生的基因的一个有吸引力的系统。
Plant-parasitic nematodes Meloidogyne spp induce an elaborate permanent feeding site characterized by the redifferentiation of root cells into multinucleate and hypertrophied giant cells. We have isolated by a promoter trap strategy an Arabidopsis thaliana formin gene, AtFH6, which is upregulated during giant cell formation. Formins are actin-nucleating proteins that stimulate de novo polymerization of actin filaments. We show here that three type-1 formins were upregulated in giant cells and that the AtFH6 protein was anchored to the plasma membrane and uniformly distributed. Suppression of the budding defect of the Saccharomyces cerevisiae bni1Delta bnr1Delta mutant showed that AtFH6 regulates polarized growth by controlling the assembly of actin cables. Our results suggest that AtFH6 might be involved in the isotropic growth of hypertrophied feeding cells via the reorganization of the actin cytoskeleton. The actin cables would serve as tracks for vesicle trafficking needed for extensive plasma membrane and cell wall biogenesis. Therefore, determining how plant parasitic nematodes modify root cells into giant cells represents an attractive system to identify genes that regulate cell growth and morphogenesis.