Mass spectrometric analysis of Plasmodium falciparum erythrocyte membrane protein-1 variants expressed by placental malaria parasites
Mass spectrometric analysis of Plasmodium falciparum erythrocyte membrane protein-1 variants expressed by placental malaria parasites
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DOI：
10.1002/pmic.200300666
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发表时间：
2004-04-01
期刊：
影响因子：
3.4
通讯作者：
Duffy, PE
中科院分区：
文献类型：
--
作者：
Fried, M;Wendler, JN;Duffy, PE
表面蛋白是重要的疟疾疫苗靶标。然而，先前鉴定的表面蛋白具有高度变异性且难以研究。我们使用串联质谱法来表征感染疟疾的红细胞表面表达的变异抗原（恶性疟原虫红细胞膜蛋白 1 (PfEMP1)），这些抗原与胎盘中的硫酸软骨素 A (CSA) 结合。虽然先前检测到与 CSA 配体有关的 PfEMP1 变体，但在未选择的寄生虫中，在 CSA 结合或胎盘寄生虫中检测到了四种新变体，但在未选择的寄生虫中未检测到。这些新的 PfEMP1 变体需要进一步研究以确认它们是否在胎盘疟疾中发挥作用。
Surface proteins from are important malaria vaccine targets. However, the surface proteins Previously identified are highly variant and difficult to study. We used tandem mass spectrometry to characterize the variant antigens (Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1)) expressed on the surface of malaria-infected erythrocytes that bind to chondroitin sulfate A (CSA) in the placenta. Whereas PfEMP1 variants previously implicated as CSA ligands were detected, in unselected parasites four novel variants were detected in CSA-binding or placental parasites but not in unselected paragites. These novel PfEMP1 variants require further study to confirm whether they play a role in placental malaria.