Highly Visible Expression of an Oxytocin-Monomeric Red Fluorescent Protein 1 Fusion Gene in the Hypothalamus and Posterior Pituitary of Transgenic Rats
Highly Visible Expression of an Oxytocin-Monomeric Red Fluorescent Protein 1 Fusion Gene in the Hypothalamus and Posterior Pituitary of Transgenic Rats
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DOI：
10.1210/en.2011-0006
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发表时间：
2011-07-01
期刊：
影响因子：
4.8
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Katoh, Akiko;Fujihara, Hiroaki;Ueta, Yoichi
我们已经产生了大鼠轴承催产素（OXT）-单体红色荧光蛋白1（mRFP 1）融合转基因。mRFP 1荧光在整个标本的视上核腹侧部和垂体后叶可见。下丘脑切片中的mRFP 1荧光也在SON、室旁核（PVN）和正中隆起的内层中观察到。盐负荷5 d引起SON、PVN、正中隆起和垂体后叶中mRFP 1荧光显著增加。原位杂交组织化学结果显示，OXT-mRFP 1融合基因在正常大鼠视上核和室旁核中表达，慢性盐负荷后表达显著增加。内源性OXT和精氨酸加压素（AVP）基因的表达显着增加，在SON和PVN慢性盐负荷后在非转基因和转基因大鼠。这些反应在雄性和雌性大鼠之间没有差异。与非转基因大鼠相比，水分充足和盐负荷的雄性和雌性转基因大鼠的血浆渗透压，钠浓度，OXT和AVP水平没有显着差异。最后，我们成功地产生了双转基因大鼠，表达OXT-mRFP 1融合基因和AVP增强的绿色荧光蛋白融合基因。我们的新转基因大鼠是研究下丘脑-神经垂体系统生理学的有价值的新工具。（内分泌学152：2768-2774，2011）
We have generated rats bearing an oxytocin (OXT)-monomeric red fluorescent protein 1 (mRFP1) fusion transgene. The mRFP1 fluorescence was highly visible in ventral part of the supraoptic nucleus (SON) and the posterior pituitary in a whole mount. mRFP1 fluorescence in hypothalamic sections was also observed in the SON, the paraventricular nucleus (PVN), and the internal layer of the median eminence. Salt loading for 5 d caused a marked increase in mRFP1 fluorescence in the SON, the PVN, the median eminence, and the posterior pituitary. In situ hybridization histochemistry revealed that the expression of the mRNA encoding the OXT-mRFP1 fusion gene was observed in the SON and the PVN of euhydrated rats and increased dramatically after chronic salt loading. The expression of the endogenous OXT and the arginine vasopressin (AVP) genes were significantly increased in the SON and the PVN after chronic salt loading in both nontransgenic and transgenic rats. These responses were not different between male and female rats. Compared with nontransgenic rats, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration, OXT, and AVP levels. Finally, we succeeded in generating a double-transgenic rat that expresses both the OXT-mRFP1 fusion gene and the AVP-enhanced green fluorescent protein fusion gene. Our new transgenic rats are valuable new tools to study the physiology of the hypothalamo-neurohypophysial system. (Endocrinology 152: 2768-2774, 2011)