PURIFICATION AND PROPERTIES OF AN INTRANUCLEAR VIRUS-SPECIFIC ANTIGEN FROM TISSUE INFECTED WITH BORNA DISEASE VIRUS
PURIFICATION AND PROPERTIES OF AN INTRANUCLEAR VIRUS-SPECIFIC ANTIGEN FROM TISSUE INFECTED WITH BORNA DISEASE VIRUS
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DOI：
10.1099/0022-1317-67-2-235
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发表时间：
1986-02-01
影响因子：
3.8
通讯作者：
ROTT, R
中科院分区：
文献类型：
--
作者：
HAAS, B;BECHT, H;ROTT, R
从博尔纳病（BD）病毒感染的大鼠脑和MDCK细胞中提取病毒特异性抗原，并通过免疫亲和层析和HPLC纯化至均一。抗原由两种大小几乎相等的组分组成（38000 mol. wt.），并且它以其天然形式形成聚集体。两种抗原实体的病毒特异性通过与恢复期血清和单克隆抗体的免疫印迹证实。用单克隆抗体和针对纯化抗原制备的超免疫血清的免疫荧光染色显示了BD病毒感染细胞的典型核内荧光。
A virus-specific antigen was extracted from brains of rats and from MDCK cells infected with Borna disease (BD) virus and purified to homogeneity by immunoaffinity chromatography and HPLC. The antigen consists of two components which are almost equal in size (38000 mol. wt.), and it forms aggregates in its native form. The virus specificity of the two antigenic entities was confirmed by immunoblots with convalescent serum and monoclonal antibodies. Immunofluorescent staining with monoclonal antibodies and a hyperimmune serum prepared against the purified antigen showed the intranuclear fluorescence typical for BD virus-infected cells.