Characterization of the cell wall of a mushroom forming fungus at atomic resolution using solid-state NMR spectroscopy.
Characterization of the cell wall of a mushroom forming fungus at atomic resolution using solid-state NMR spectroscopy.
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DOI：
10.1016/j.tcsw.2020.100046
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Baldus M
中科院分区：
文献类型：
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作者：
Ehren HL;Appels FVW;Houben K;Renault MAM;Wösten HAB;Baldus M
细胞壁在真菌与生物环境的相互作用中是必不可少的，也是菌丝形态发生和机械强度的关键。在这里，我们使用固态NMR（ssNMR）光谱结合HPLC和GC-MS研究的代表性的担子菌，真菌的两个主要门之一的细胞壁的结构组织。基于这些数据，我们提出了一个改进的模型为细胞壁的一个担子菌。在该模型中，刚性核心由α-和β-（1，3）-葡聚糖、β-（1，3）-（1，6）-葡聚糖、高度支化和单链β-（1，4）-几丁质以及聚合岩藻糖构建。细胞壁的移动的部分由β-（1，3）-葡聚糖、β-（1，3）-（1，6）-葡聚糖、β-（1，6）-葡聚糖、α-连接的还原和非还原末端以及聚合甘露糖组成。总之，这些发现提供了新的见解模型担子菌S细胞壁的结构组织。以前基于破坏性的化学和酶分析的公社。
Cell walls are essential in the interaction of fungi with the (a)biotic environment and are also key to hyphal morphogenesis and mechanical strength. Here, we used solid-state NMR (ssNMR) spectroscopy combined with HPLC and GC–MS to study the structural organization of the cell wall of a representative of the Basidiomycota, one of the two main phyla of fungi. Based on the data we propose a refined model for the cell wall of a basidiomycete. In this model, the rigid core is built from α- and β-(1,3)-glucan, β-(1,3)-(1,6)-glucan, highly branched and single stranded β-(1,4)-chitin as well as polymeric fucose. The mobile fraction of the cell wall is composed of β-(1,3)-glucan, β-(1,3)-(1,6)-glucan, β-(1,6)-glucan, α-linked reducing and non-reducing ends and polymeric mannose. Together, these findings provide novel insights into the structural organization of the cell wall of the model basidiomycete S. commune that was previously based on destructive chemical and enzymatic analysis.