Long-term ingestion of insoluble dietary fiber increases luminal mucin content, but has no effect on nutrient absorption in rats
Long-term ingestion of insoluble dietary fiber increases luminal mucin content, but has no effect on nutrient absorption in rats
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DOI：
10.1271/bbb.70643
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发表时间：
2008-03-01
影响因子：
1.6
通讯作者：
Kiriyama, Shuhachi
中科院分区：
文献类型：
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作者：
Morita, Tatsuya;Tanabe, Hiroki;Kiriyama, Shuhachi
我们重新验证了摄入不溶性膳食纤维(IDF)导致粘蛋白分泌增加会影响小肠养分吸收的假说。使用聚苯乙烯泡沫塑料(PSF)作为IDF。大鼠分别饲喂含或不含PSF/kg的饲料1、2、4wk。在每个周期结束时，在食物耗尽12h后插管葡萄糖和卵清蛋白(OVA)溶液，并监测这些成分的血清浓度变化。用O-连接的寡糖链测定鲁米那粘蛋白，并用酶联免疫吸附试验测定。在所有时期，饲喂PSF组的管腔粘蛋白含量均高于无纤维对照组。然而，在任何时期的任何时间，两组之间的血糖和卵清蛋白浓度的变化是相似的。这些结果表明，即使在慢性摄入IDF后，管腔粘蛋白分泌的增加仍然持续，但管腔粘蛋白含量的增加对管腔养分的吸收速度没有影响。
We reexamined the hypothesis that increased mucin secretion by the ingestion of insoluble dietary fiber (IDF) could affect small intestinal nutrient absorption. Polystyrene foam (PSF) was used as IDF. Rats were fed a diet with or without 90 g of PSF/kg for 1, 2 and 4 wk. At the end of each period, a glucose and ovalbumin (OVA) solution was intubated after 12h of food depletion, and the changes in serum concentrations of these components were monitored. Luminal mucin was measured as O-linked oligosaccharide chains and also determined by ELISA. In all periods, the luminal mucin content was greater in the PSF-fed group than in the fiber-free control. However, the changes in serum glucose and OVA concentrations were comparable between the groups at any time during any period. These results show that the enhancement of luminal mucin secretion lasted even after chronic ingestion of IDF, but that the increased luminal mucin content had no effect on the rate of luminal nutrient absorption.