Measurements of catecholamine-mediated apoptosis of immunocompetent cells by capillary electrophoresis
Measurements of catecholamine-mediated apoptosis of immunocompetent cells by capillary electrophoresis
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DOI：
10.1002/elps.1150181009
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发表时间：
1997-09-01
期刊：
影响因子：
2.9
通讯作者：
Ewing, A
中科院分区：
文献类型：
--
作者：
Bergquist, J;Josefsson, E;Ewing, A
毛细管电泳单细胞分析技术是一种能够检测神经化学物质齐托莫尔量(10(-21)摩尔)的技术，已被用来证明淋巴细胞能够活跃地合成多巴胺和去甲肾上腺素。淋巴细胞暴露于浓度低至10 NM的儿茶酚胺会导致细胞增殖和分化减少，如干扰素-伽马、白介素4和免疫球蛋白。此外，诺米芬新抑制多巴胺摄取和四苯那嗪抑制儿茶酚胺进入囊泡均导致多巴胺和去甲肾上腺素水平显著降低(分别为p<；0.01和p<；0.05)。儿茶酚胺对T和B淋巴细胞活性的抑制是通过诱导参与细胞凋亡的Bcl2/Bax和Fas/FasL来实现的。这些发现表明了一种调节中枢神经系统中淋巴细胞活性的新机制，即局部儿茶酚胺水平的升高可能导致大脑的免疫特权。
Single cell analysis with capillary electrophoresis, a technique capable of detecting zeptomole quantities (10(-21) mole) of neurochemical species, has been used to demonstrate that lymphocytes are capable of active synthesis of dopamine and norepinephrine. Exposure of lymphocytes to catecholamines at concentrations as low as 10 nM leads to decreased proliferation and differentiation, e.g. interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and immunoglobulin (Ig). In addition, both inhibition of dopamine uptake with nomifensine and inhibition of packing of catecholamines into vesicles with tetrabenazine, results in significantly lower levels of dopamine and norepinephrine (p < 0.01 and p < 0.05, respectively). The catecholamine-dependent inhibition of T-and B-lymphocyte activity is mediated via an induction of a Bcl-2/Bax and Fas/FasL involved apoptosis. These findings indicate a novel mechanism for regulation of lymphocyte activity in the central nervous system, whereby elevated regional levels of catecholamines might lead to the immunoprivilege of the brain.