Expression and distribution of all dopamine receptor subtypes (D1-D5) in the mouse lumbar spinal cord:: A real-time polymerase chain reaction and non-autoradiographic in situ hybridization study
Expression and distribution of all dopamine receptor subtypes (D1-D5) in the mouse lumbar spinal cord:: A real-time polymerase chain reaction and non-autoradiographic in situ hybridization study
复制标题
DOI：
10.1016/j.neuroscience.2007.07.052
复制
发表时间：
2007-11-23
期刊：
影响因子：
3.3
通讯作者：
Hochman, S.
中科院分区：
文献类型：
--
作者：
Zhu, H.;Clemens, S.;Hochman, S.
多巴胺是一种儿茶酚胺能神经调节递质，通过五种分子上不同的G蛋白偶联受体亚型（D-1-D-5）发挥作用。在哺乳动物脊髓中，多巴胺能轴突侧支主要来自下丘脑背后部的All区域，并在整个脊髓神经轴中弥漫性投射。多巴胺能调节作用涉及脊髓中的感觉、运动和自主神经功能，但脊髓中不同多巴胺受体的表达特性仍然不完整。在这里，我们通过定量的真实的聚合酶链反应（PCR）和洋地黄毒苷标记的原位杂交来确定小鼠脊髓细胞中所有多巴胺受体亚型的存在和区域分布。实时PCR表明，所有多巴胺受体表达在脊髓中具有强烈的显性D2受体表达，包括在运动神经元和感觉编码浅表背角（SDH）。激光捕获显微切割（LCM）证实了SDH和运动神经元中D2受体表达的优势。腰髓的原位杂交显示，所有多巴胺受体的表达主要是在灰质，包括运动神经元，并广泛分布在标记的细胞亚群中的大多数或所有板层。观察到D2和D1受体的细胞标记发生率最高，而D1和D3受体表达的发生率最低。我们的结论是，所有已知的多巴胺受体在脊髓中的表达和广泛的突触后分布，以及与广泛的下行多巴胺能投射领土支持广泛的多巴胺能控制脊髓神经元系统。D2受体的显性表达表明这些受体在突触后脊髓神经元的多巴胺能作用中起主导作用。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Dopamine is a catecholaminergic neuromodulatory transmitter that acts through five molecularly-distinct G protein-coupled receptor subtypes (D-1-D-5). In the mammalian spinal cord, dopaminergic axon collaterals arise predominantly from the All region of the dorsoposterior hypothalamus and project diffusely throughout the spinal neuraxis. Dopaminergic modulatory actions are implicated in sensory, motor and autonomic functions in the spinal cord but the expression properties of the different dopamine receptors in the spinal cord remain incomplete. Here we determined the presence and the regional distribution of all dopamine receptor subtypes in mouse spinal cord cells by means of quantitative real time polymerase chain reaction (PCR) and digoxigenin-label in situ hybridization. Real-time PCR demonstrated that all dopamine receptors are expressed in the spinal cord with strongly dominant D2 receptor expression, including in motoneurons and in the sensory encoding superficial dorsal horn (SDH). Laser capture microdissection (LCM) corroborated the predominance of D2 receptor expression in SDH and motoneurons. In situ hybridization of lumbar cord revealed that expression for all dopamine receptors was largely in the gray matter, including motoneurons, and distributed diffusely in labeled cell subpopulations in most or all laminae. The highest incidence of cellular labeling was observed for D2 and D, receptors, while the incidence of D, and D-3 receptor expression was least. We conclude that the expression and extensive postsynaptic distribution of all known dopamine receptors in spinal cord correspond well with the broad descending dopaminergic projection territory supporting a widespread dopaminergic control over spinal neuronal systems. The dominant expression of D2 receptors suggests a leading role for these receptors in dopaminergic actions on postsynaptic spinal neurons. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.