Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin
Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin
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DOI：
10.1016/j.taap.2005.08.008
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发表时间：
2006-05-15
影响因子：
3.8
通讯作者：
Liu, Cheng-Tzu
中科院分区：
文献类型：
--
作者：
Chiang, Yi-Hsuan;Jen, Lin-Ni;Liu, Cheng-Tzu
大蒜及其活性成分具有抗氧化和抗衰老的作用。本研究探讨了大蒜油及其有机硫化合物对内毒素诱导的肠粘膜损伤的影响。Wistar大鼠在注射内毒素（i. p.，5 mg/kg体重）。对照组大鼠给予玉米油，并注射无菌生理盐水。在注射后3小时收集用于测量促炎性细胞因子的样品，并且在注射后18小时收集所有其他样品。低剂量GO可抑制内毒素诱导的肠黏膜诱导型一氧化氮合酶（iNOS）活性、溃疡形成和细胞凋亡（P < 0.05）。高剂量GO可显著降低外周血NO/NO水平和内毒素诱导的肠粘膜NOS活性（P < 0.05），但可加重肠粘膜损伤，并伴有外周血促炎细胞因子的升高。二烯丙基三硫化物而非二烯丙基二硫化物显示出与高剂量GO相似的毒性作用。这些结果表明大蒜油及其有机硫化合物对内毒素诱导的全身炎症和肠道损伤具有预防作用和可能的毒性。(c)2005年爱思唯尔公司All rights reserved.
Garlic and its active components are known to possess antioxidant and antiinflammatory effects. The present study investigated the effects of garlic oil and its organosulfur compounds on endotoxin-induced intestinal mucosal damage. Wistar rats received by gavage 50 or 200 mg/kg body weight garlic oil (GO), 0.5 mmol/kg body weight diallyl disulfide or diallyl trisulfide, or the vehicle (corn oil; 2 ml/kg body weight) every other day for 2 weeks before being injected with endotoxin (i.p., 5 mg/kg body weight). Control rats were administered with corn oil and were injected with sterile saline. Samples for the measurement of proinflammatory cytokines were collected 3 h after injection, and all other samples were collected 18 h after injection. The low dose of GO suppressed endotoxin-induced inducible nitric oxide synthase (iNOS) activity, ulceration, and apoptosis in the intestinal mucosa (P < 0.05). The high dose of GO significantly lowered the peripheral level of nitrate/nitrite and endotoxin-induced NOS activity in the intestinal mucosa (P < 0.05) but worsened intestinal mucosal damage accompanied by elevated peripheral proinflammatory cytokines. Diallyl trisulfide but not diallyl disulfide showed similar toxic effect as that of high-dose GO. These results suggest the preventive effect and possible toxicity of garlic oil and its organosulfur compounds in endotoxin-induced systemic inflammation and intestinal damage. (c) 2005 Elsevier Inc. All rights reserved.