Suppression of the floral activator Hd3a is the principal cause of the night break effect in rice
Suppression of the floral activator Hd3a is the principal cause of the night break effect in rice
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DOI：
10.1105/tpc.105.037028
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发表时间：
2005-12-01
期刊：
影响因子：
11.6
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Ishikawa, R;Tamaki, S;Shimamoto, K
在半夜短时间暴露在光下会抑制短日照植物的开花。这种现象被称为夜间休息（NB），多年来一直被广泛用作研究开花的光周期控制的工具。然而，在分子水平上，人们对这种现象知之甚少。在水稻（水稻）中，在14小时的夜晚中曝光10分钟会导致开花明显延迟。单个NB强烈抑制Hd3a的mRNA，Hd3a是拟南芥FLOW LOCUS T（FT）的同源物，而OsGI和Hd1的mRNA不受影响。NB对Hd3a mRNA的影响在14 h的半夜达到最大。phyB突变体消除了NB对开花和Hd3 a mRNA的影响，表明NB效应是由光敏色素B介导的。由于其他FT样基因的表达量很低，没有受到NB的明显影响，我们的研究结果强烈表明，Hd3a mRNA的抑制是NB影响水稻开花的主要原因。
A short exposure to light in the middle of the night causes inhibition of flowering in short-day plants. This phenomenon is called night break (NB) and has been used extensively as a tool to study the photoperiodic control of flowering for many years. However, at the molecular level, very little is known about this phenomenon. In rice (Oryza sativa), 10 min of light exposure in the middle of a 14-h night caused a clear delay in flowering. A single NB strongly suppressed the mRNA of Hd3a, a homolog of Arabidopsis thaliana FLOWERING LOCUS T (FT), whereas the mRNAs of OsGI and Hd1 were not affected. The NB effect on Hd3a mRNA was maximal in the middle of the 14-h night. The phyB mutation abolished the NB effect on flowering and Hd3a mRNA, indicating that the NB effect was mediated by phytochrome B. Because expression of the other FT-like genes was very low and not appreciably affected by NB, our results strongly suggest that the suppression of Hd3a mRNA is the principal cause of the NB effect on flowering in rice.