A linear dilution microfluidic device for cytotoxicity assays
A linear dilution microfluidic device for cytotoxicity assays
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DOI：
10.1039/b608990a
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
O'Neill, Adrian T.
中科院分区：
文献类型：
--
作者：
Walker, Glenn M.;Monteiro-Riviere, Nancy;O'Neill, Adrian T.
提出了一种双层聚合物微流体装置，该装置从以不同体积比例混合的两种输入流体流中产生九种线性稀释液。当流速保持恒定在 1.0 μ l min(-1) (R-2 = 0.9995) 和当流速在 0.5-16 μ l min(-1) (R-2 = 0.9998) 变化时，九种稀释度的线性度保持不变。提出了用于设计具有任意数量的线性稀释的微流体装置的解析表达式。为了证明该装置的功效，用九种稀释度的钙黄绿素对原代人表皮角质形成细胞 (HEK) 进行染色，从而产生荧光强度的线性分布 (R-2 = 0.94)。该装置的操作原理可以扩大规模以合并任意数量的线性稀释。这种可扩展性，加上在各种操作条件下产生线性稀释的内在能力，使得该设备适用于高通量筛选应用，例如组合化学或细胞毒性测定。
A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 mu l min(-1) (R-2 = 0.9995) and when it is varied from 0.5-16 mu l min(-1) (R-2 = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R-2 = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays.