Glucose Uptake as an Alternative to Stop-Flow Respirometry for Measuring Metabolic Rate in Danio rerio Larvae
Glucose Uptake as an Alternative to Stop-Flow Respirometry for Measuring Metabolic Rate in Danio rerio Larvae
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DOI：
10.1101/2021.04.30.442098
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发表时间：
2021-04
期刊：
影响因子：
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通讯作者：
B. L. Evans;A. Hurlstone;P. Clayton;A. Stevens;H. Shiels
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作者：
B. L. Evans;A. Hurlstone;P. Clayton;A. Stevens;H. Shiels
呼吸测定法是目前测量代谢率的金标准。然而，对于适合发育研究的代谢率测量的需求越来越大，特别是在小于4 mm的斑马鱼中，许多重要的发育阶段发生。虽然许多代谢研究依赖于呼吸测量，但设备的成本和复杂性限制了其在非专业实验室中的吸引力，并且随着生物体尺寸的减小，背景呼吸变得越来越成问题。在此，将葡萄糖摄取与停流呼吸测定法进行了比较，作为更适合于发育研究所需的小规模代谢率的替代测量方法。Passing-Bablok回归分析显示，在95%的一致性限度内，葡萄糖摄取率可视为等同于耗氧量，作为斑马鱼幼虫代谢率的指标。因此，在常规呼吸测定法存在问题的小生物体中，葡萄糖摄取是金标准的有效替代方法。总结声明葡萄糖摄取率是一个有效的替代呼吸测定代谢率测量小鱼。
Respirometry is the current gold-standard for measuring metabolic rate. However, there is a growing need for metabolic rate measurements suitable for developmental studies, particularly in Danio rerio, where many important developmental stages occur at < 4 mm. While many metabolic studies rely on respirometry, the cost and complexity of the equipment limits its appeal in non-specialist labs, and background respiration becomes increasingly problematic as the size of the organism reduces. Here, glucose uptake was compared to stop-flow respirometry as an alternative measure of metabolic rate more suitable to the small scale required for developmental studies. A Passing-Bablok regression revealed the rate of glucose uptake can be considered equivalent to oxygen consumption as a measure of metabolic rate in Danio rerio larvae within a 95% limit of agreement. Therefore, glucose uptake is a valid alternative to the gold-standard in small organisms where conventional respirometry is problematic. Summary statement The rate of glucose uptake is a valid alternative to respirometry for metabolic rate measurements in small larval fish.