Genome-wide identification and expression analysis of rice cell cycle genes
Genome-wide identification and expression analysis of rice cell cycle genes
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DOI：
10.1007/s11103-007-9154-y
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发表时间：
2007-07-01
影响因子：
5.1
通讯作者：
Zhang, Xian Sheng
中科院分区：
文献类型：
--
作者：
Guo, Jing;Song, Jian;Zhang, Xian Sheng
细胞周期蛋白、细胞周期蛋白依赖性激酶和许多其他蛋白质控制植物细胞周期的进程。虽然大量的研究揭示了拟南芥中一些细胞周期调控因子的作用及其机制，但相对较少的细胞周期调控因子在水稻中进行了功能分析。在这项研究中，我们描述了水稻基因组中的41个调节子。我们的研究结果表明，水稻基因组包含的核心细胞周期调控因子的数量比拟南芥少，虽然水稻基因组比拟南芥的大得多。通过系统发育分析，在水稻基因组中发现了8组与拟南芥相似的CDKs，分别对应于E2 F、CKI、Rb、CKS和Wee。核心细胞调节因子的结构在水稻和拟南芥基因组中相对保守。此外，大多数的核心细胞周期基因的表达空间调控，最密切相关的表现出非常相似的模式的表达，表明功能冗余和高度相似的核心细胞周期基因之间的保护在水稻和拟南芥。生长素或细胞分裂素处理后，核心细胞周期基因的表达上调或下调，表明生长素和/或细胞分裂素可能直接调节核心细胞周期基因的表达。本研究结果为深入了解水稻细胞周期调控机制和细胞周期相关基因的功能提供了基础信息。
Cyclins, cyclin-dependent kinases, and a number of other proteins control the progression of plant cell cycle. Although extensive studies have revealed the roles of some cell cycle regulators and the underlying mechanisms in Arabidopsis, relatively a small number of cell cycle regulators were functionally analyzed in rice. In this study, we describe 41 regulators in the rice genome. Our results indicate that the rice genome contains a less number of the core cell cycle regulators than the Arabidopsis one does, although the rice genome is much larger than the Arabidopsis one. Eight groups of CDKs similar to those in Arabidopsis were identified in the rice genome through phylogenetic analysis, and the corresponding members in the different groups include E2F, CKI, Rb, CKS and Wee. The structures of the core cell regulators were relatively conserved between the rice and Arabidopsis genomes. Furthermore, the expression of the majority of the core cell cycle genes was spatially regulated, and the most closely related ones showed very similar patterns of expression, suggesting functional redundancy and conservation between the highly similar core cell cycle genes in rice and Arabidopsis. Following auxin or cytokinin treatment, the expression of the core cell cycle genes was either upregulated or downregulated, suggesting that auxin and/or cytokinin may directly regulate the expression of the core cell cycle genes. Our results provide basic information to understand the mechanism of cell cycle regulation and the functions of the rice cell cycle genes.