GATA3 siRNA inhibits the binding of NFAT1 to interleukin-13 promoter in human T cells.
GATA3 siRNA inhibits the binding of NFAT1 to interleukin-13 promoter in human T cells.
复制标题
GATA3 siRNA 抑制人 T 细胞中 NFAT1 与 IL-13 启动子的结合。
DOI：
10.3760/cma.j.issn.0366-6999.2010.06.019
复制
发表时间：
2010
影响因子：
6.1
通讯作者：
Mao Huang
中科院分区：
文献类型：
--
作者：
X. Yao;Yan Yang;Hai;Min Wang;K. Yin;Mao Huang
BACKGROUND
Interleukin-13 (IL-13) is recognized to be a key modulator in the pathogenesis of Th2-induced allergic inflammation. Transcription factors GATA3 and NFAT1 have been both implicated in the regulation of Th2 cytokines. We previously demonstrated the GATA3-NFAT1 association during human T cell activation. However, the function of the GATA3-NFAT1 complex in Th2 cytokines regulation is still unknown. Small interference RNA (siRNA) was constructed to knock down GATA3 expression in Hut-78 cells to investigate the possible role of GATA3-NFAT1 complex in IL-13 transcription.
METHODS
Cells were stimulated with anti-CD3 plus anti-CD28 antibodies to mimic in vivo antigen-mediated co-stimulation; the expression of IL-13 mRNA was determined by real-time PCR; chromation immunoprecipitation (CHIP) assay was employed to investigate the NFAT1 binding to IL-13 promoter.
RESULTS
GATA3 siRNA suppressed the expression of GATA3 both in mRNA and protein levels in Hut-78 cells. The binding of NFAT1 to IL-13 promoter was inhibited by GATA3 siRNA in activated T cells, which was followed by the reduction of IL-13 transcription.
CONCLUSION
GATA3-NFAT1 complex may play an important role in the regulation of IL-13 transcription in human T cells.
缺乏转录因子 NFAT1 的小鼠过敏性炎症和嗜酸性粒细胞募集的调节：白细胞介素 4 (IL-4) 和 IL-5 的作用。
影响因子：
20.3
作者：
Viola,JP;Kiani,A;Bozza,PT;Rao,A
通讯作者：
Rao,A