Comprehensive Analysis of the COBRA-Like (COBL) Gene Family in Gossypium Identifies Two COBLs Potentially Associated with Fiber Quality.
Comprehensive Analysis of the COBRA-Like (COBL) Gene Family in Gossypium Identifies Two COBLs Potentially Associated with Fiber Quality.
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对棉中 COBRA 样 (COBL) 基因家族的综合分析确定了两个可能与纤维质量相关的 COBL
DOI：
10.1371/journal.pone.0145725
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Niu E;Shang X;Cheng C;Bao J;Zeng Y;Cai C;Du X;Guo W
COBRA-Like（COBL）基因编码一种植物特有的糖基磷脂酰肌醇（GPI）锚定蛋白，是细胞生长方向和纤维素结晶状态的关键调控因子。全基因组分析已在A. thaliana、拟南芥O. sativa，Z.梅斯和S.番茄属中，而棉属中很少。在此，我们从三个已测序的棉花物种中鉴定了19、18和33个候选COBL基因，二倍体棉花G。Raimondii，G. arboreum和四倍体棉G. hirsutum acc. TM-1。这些COBL成员锚定在G.雷蒙地亚属又可分为两个亚组。COBL基因在G.多毛草TM-1.其中，GhCOBL 9和GhCOBL 13在纤维发育的次生细胞壁阶段优先表达，并与纤维素合成酶基因GhCESA 4、GhCESA 7和GhCESA 8显著共上调表达。此外，GhCOBL 9 Dt和GhCOBL 13 Dt与已报道的棉花纤维品质QTL共定位，且GhCOBL 9 Dt的有利等位基因类型与纤维品质性状呈显著正相关，表明这两个基因可能在纤维发育中起重要作用。
COBRA-Like (COBL) genes, which encode a plant-specific glycosylphosphatidylinositol (GPI) anchored protein, have been proven to be key regulators in the orientation of cell expansion and cellulose crystallinity status. Genome-wide analysis has been performed in A. thaliana, O. sativa, Z. mays and S. lycopersicum, but little in Gossypium. Here we identified 19, 18 and 33 candidate COBL genes from three sequenced cotton species, diploid cotton G. raimondii, G. arboreum and tetraploid cotton G. hirsutum acc. TM-1, respectively. These COBL members were anchored onto 10 chromosomes in G. raimondii and could be divided into two subgroups. Expression patterns of COBL genes showed highly developmental and spatial regulation in G. hirsutum acc. TM-1. Of them, GhCOBL9 and GhCOBL13 were preferentially expressed at the secondary cell wall stage of fiber development and had significantly co-upregulated expression with cellulose synthase genes GhCESA4, GhCESA7 and GhCESA8. Besides, GhCOBL9 Dt and GhCOBL13 Dt were co-localized with previously reported cotton fiber quality quantitative trait loci (QTLs) and the favorable allele types of GhCOBL9 Dt had significantly positive correlations with fiber quality traits, indicating that these two genes might play an important role in fiber development.