High resolution gas chromatography of urinary steroids on glass capillary columns.
High resolution gas chromatography of urinary steroids on glass capillary columns.
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玻璃毛细管柱上尿类固醇的高分辨率气相色谱法。
DOI：
10.1016/0009-8981(71)90173-2
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. Völlmin
中科院分区：
文献类型：
--
作者：
J. Völlmin
建立了玻璃毛细管柱气相色谱测定尿中类固醇类化合物的方法。这些类固醇被分离为三甲基硅基或甲氧肟-三甲基硅基醚衍生物。通过这种方式，可以在不进行任何预纯化步骤的情况下获得尿类固醇的分布。具有诊断价值的类固醇(雄酮、黄酮醇、脱氢表雄酮、11-酮基胆汁酮、孕二醇、孕三醇酮、四氢S和四氢E)可在同一色谱图中定量测定。与其他特定方法相比，该方法可以快速识别类固醇代谢障碍(例如，区分各种类型的先天性肾上腺增生症)。
A gas chromatographic method for the determination of urinary steroids on glass capillary columns is described. The steroids are separated as trimethylsilyl or methoxime-trimethylsilyl ether derivatives. In this way it is possible to obtain a profile of urinary steroids without any prepurification steps. Steroids of diagnostic importance (androsterone, etiocholanolone, dehydroepiandrosterone, 11-keto-etiocholanolone, pregnanediol, pregnanetriol, pregnenetriol, pregnanetriolone, tetrahydro S and tetrahydro E) can be determined quantitatively in the same chromatogram. Compared to other specific methods, this procedure allows a fast recognition of disorders of the steroid metabolism (e.g. the differentiation of various types of congenital adrenal hyperplasia).