Photostable and Biocompatible Luminescent Thiol-Terminated Organosilica Nanoparticles with Embedded Au(I)-Thiolate Complexes for Fluorescent Microscopic Imaging
Photostable and Biocompatible Luminescent Thiol-Terminated Organosilica Nanoparticles with Embedded Au(I)-Thiolate Complexes for Fluorescent Microscopic Imaging
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DOI：
10.1021/acsanm.1c02826
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发表时间：
2021-12-13
影响因子：
5.9
通讯作者：
Nakamura, Michihiro
中科院分区：
文献类型：
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作者：
Mochizuki, Chihiro;Nakamura, Junna;Nakamura, Michihiro
荧光二氧化硅纳米颗粒（NPs）由于其高荧光强度和光稳定性、多功能性和生物相容性，在荧光显微镜（FM）中具有很大的应用前景。通常，荧光二氧化硅NPs是由二氧化硅和荧光有机染料或无机材料合成的。本研究重点研究了Au(I)配合物在特定条件下表现出的与过渡金属配合物明显不同的光致发光机制。绿色合成条件下制备的嵌入Au(I)-硫酸盐配合物（thiol-OS/Au）的发光硫醇端有机二氧化硅NPs维持了Au的PL态。此外，硫醇os /Au的高Au含量使其发射出可被FM成像检测到的红色PL，在约700 nm处显示出具有大Stokes位移的PL发射峰。巯基os /Au具有高度的生物相容性，pH稳定（pH 2-12），适用于长期细胞成像。在30分钟的激发后，用硫醇os /Au标记的细胞没有出现光漂白，这鼓励使用硫醇os /Au作为FM生物成像的稳定荧光探针。
Fluorescent silica nanoparticles (NPs) hold great promise in fluorescence microscopy (FM) owing to their high fluorescence intensity and photostability, multifunctionality, and biocompatibility. Conventionally, fluorescent silica NPs are synthesized from silica and fluorescent organic dyes or inorganic materials. This study focuses on photoluminescence (PL) exhibited by Au(I) complexes under specific conditions through a mechanism that is significantly different from that of the PL exhibited by transition-metal complexes. Luminescent thiol-terminated organosilica NPs with embedded Au(I)-thiolate complexes (thiol-OS/Au) produced under green synthetic conditions sustained the PL state of Au. Furthermore, the high Au content of thiol-OS/Au caused it to emit red PL detectable by FM imaging, exhibiting a PL emission peak at approximately 700 nm with a large Stokes shift. The size-tunable thiol-OS/Au was highly biocompatible, pH-stable (pH 2-12), and applicable to long-term cell imaging. Cells labeled using thiol-OS/Au did not exhibit photobleaching, following 30 min of excitation, encouraging the use of thiol-OS/Au as a stable fluorescent probe for FM bioimaging.